{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T21:49:05Z","timestamp":1792187345579,"version":"4.4.0"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,3,7]],"date-time":"2024-03-07T00:00:00Z","timestamp":1709769600000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["ascpt.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Translational Sci"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Ankylosing spondylitis (AS) is an immune chronic inflammatory disease, resulting in back pain, stiffness, and thoracolumbar kyphotic deformity. Based on the reported anti\u2010inflammatory and antioxidant capacities of cynarin (Cyn), this study explored its protective role and molecular mechanisms in mesenchymal stem cells (MSCs) from AS. The target pathways and genes were verified using Western blotting, quantitative real\u2010time polymerase chain reaction, and immunofluorescent staining, while molecular docking analysis was conducted. In AS\u2010MSCs, we found that the expression levels of p\u2010NF\u2010\u03baB, IL\u20106, IL\u20101\u03b2, and TNF\u2010\u03b1 were higher and I\u03baB\u2010\u03b1, Nrf\u20102, and HO\u20101 were lower compared with healthy control (HC)\u2010MSCs. With molecular docking analysis, the biding affinities between Cyn and Keap1\u2010Nrf\u20102 and p65\u2010I\u03baB\u2010\u03b1 were predicted. The mRNA and protein expression of p\u2010NF\u2010\u03baB, IL\u20106, IL\u20101\u03b2, and TNF\u2010\u03b1 and the reactive oxygen species (ROS) generation were downregulated following Cyn administration. Meanwhile, the expression level of I\u03baB\u2010\u03b1, Nrf\u20102, and HO\u20101 were significantly increased after Cyn pretreatment. The results suggested that the protective mechanisms of Cyn in AS\u2010MSCs were based on enhancing the antioxidation and suppression of excessive inflammatory responses via Nrf\u20102\/ROS\/NF\u2010\u03baB axis. Our findings demonstrate that Cyn is a potential candidate for alleviating inflammation in AS.<\/jats:p>","DOI":"10.1111\/cts.13748","type":"journal-article","created":{"date-parts":[[2024,3,7]],"date-time":"2024-03-07T06:40:15Z","timestamp":1709793615000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Nrf\u20102\/ROS\/NF\u2010\u03baB pathway is modulated by cynarin in\u00a0human mesenchymal stem cells in\u00a0vitro from ankylosing\u00a0spondylitis"],"prefix":"10.1111","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4624-4214","authenticated-orcid":false,"given":"Chenyu","family":"Song","sequence":"first","affiliation":[{"name":"Division of Spine Surgery, Department of Orthopedic Surgery Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University  Nanjing 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