{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T19:23:28Z","timestamp":1789154608453,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T00:00:00Z","timestamp":1750032000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20GM103549"],"award-info":[{"award-number":["P20GM103549"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P30GM118247"],"award-info":[{"award-number":["P30GM118247"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ascpt.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Translational Sci"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Metabolic dysfunction\u2010associated liver disease (MASLD) is a leading cause of liver disease in children. There is a paucity of data on potential biomarkers and therapeutic targets, especially in pediatric MASLD. We used mass spectrometry (MS)\u2010mediated proteomics followed by enzyme\u2010linked immunosorbent assay (ELISA) to identify potential biomarkers and therapeutic targets in pediatric MASLD. Serum samples were collected from pediatric subjects without (<jats:italic>n<\/jats:italic>\u2009=\u200956) and with MASLD (<jats:italic>n<\/jats:italic>\u2009=\u200972). Initial screen using MS\u2010based proteomics identified 6 upregulated (adenosine deaminase 2, sex hormone\u2010binding globulin (SHBG), inter\u2010alpha\u2010trypsin inhibitor heavy chain H1 (ITIH1), fructose\u2010bisphosphate aldolase A, type II cytoskeletal 2 epidermal keratine, N\u2010acetylmuramoyl\u2010L\u2010alanine amidase) and 3 downregulated (alcohol dehydrogenase 4 (ADH4), fructose\u2010bisphosphate aldolase B (ALDOB), serum albumin) proteins in the MASLD group. Confirmatory studies using ELISA were performed for the 2 strongest upregulated proteins (SHBG and ITIH1) and two top downregulated proteins (ADH4 and ALDOB). Correlation of ELISA results with clinical data revealed that SHBG had strong associations with BMI, ALT, and HgbA1c (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). ADH4 had strong associations with BMI and HgbA1c (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). ITIH1 and ALDOB had no strong correlations with common clinical parameters of MASLD. Area under ROC Curve revealed statistically significant ability of SHBG (494\u2009nmol\/L, sensitivity\u2009=\u200998%, specificity 80%) and ADH4 (2.14\u2009ng\/mL, sensitivity\u2009=\u200965%, specificity\u2009=\u200966%) to diagnosis MASLD (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). MS with confirmation ELISA identified SHBG and ADH4 as potential biomarkers of pediatric MASLD.<\/jats:p>","DOI":"10.1111\/cts.70271","type":"journal-article","created":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T13:53:39Z","timestamp":1750082019000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Proteomics Used in Identifying Novel Correlates of Disease in Pediatric Metabolic Dysfunction\u2010Associated Steatotic Liver Disease"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9047-9497","authenticated-orcid":false,"given":"Diego","family":"Paine\u2010Cabrera","sequence":"first","affiliation":[{"name":"Department of Pharmacology Toxicology and Therapeutics, University of Kansas Medical Center  Kansas City Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa K.","family":"Harvey","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology Hepatology, and Nutrition, Children's Mercy\u2014Kansas City  Kansas City Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele T.","family":"Pritchard","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Toxicology and Therapeutics, University of Kansas Medical Center  Kansas City Kansas USA"},{"name":"University of Kansas Liver Center  Kansas City Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7920-7466","authenticated-orcid":false,"given":"John","family":"Thyfault","sequence":"additional","affiliation":[{"name":"Department of Molecular and Integrative Physiology University of Kansas Medical Center  Kansas City Kansas USA"},{"name":"Children's Center for Healthy Lifestyles and Nutrition  Kansas City Missouri 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