{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T12:10:29Z","timestamp":1782303029024,"version":"3.54.5"},"reference-count":13,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["ascpt.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Translational Sci"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Paclitaxel (Taxol) is a widely used anticancer agent that undergoes extensive hepatic metabolism and that causes a debilitating, dose\u2010limiting peripheral neurotoxicity. We previously reported that the uptake of paclitaxel in hepatocytes and dorsal root ganglion neurons, the site of injury within the nervous system, is mediated by the organic anion transporting polypeptides OATP1B1 and OATP1B3 (Oatp1b2 in rodents), transporters that are highly sensitive to pharmacological inhibition. To facilitate future screens of chemical libraries to identify modulators and imaging\u2010based drug distribution studies, we explored the utility of PB\u2010Gly\u2010Taxol and PB\u2010GABA\u2010Taxol, derivatives of paclitaxel linked to the coumarin\u2010derived fluorophore Pacific Blue, as in\u00a0vitro and in\u00a0vivo substitute biomarker probes of paclitaxel. Transport studies in transfected HEK293 cells revealed efficient uptake of these PB\u2010taxoids by human and murine OATP1B\/Oatp1b\u2010type transporters, with up to 100\u2010fold increases in uptake relative to values observed in vector control cells, and inhibition of this transport by known inhibitors. Although cell viability assays demonstrated lower cytotoxicity of both PB\u2010taxoids (IC\n                    <jats:sub>50<\/jats:sub>\n                    : 13~80\u2009nM) against a panel of breast cancer cell lines, ensuing investigations confirmed their ability to induce peripheral neurotoxicity phenotypes in mice (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), in an Oatp1b2\u2010dependent manner, to the same extent as paclitaxel. These findings imply that PB\u2010taxoids mimic the transport and toxicokinetic features of paclitaxel, and these agents thus offer potential as fluorescent imaging tools for exploring drug\u2013drug interaction liabilities and paclitaxel\u2010related toxicity profiles that involve OATP1B\/Oatp1b\u2010type transporters.\n                  <\/jats:p>","DOI":"10.1111\/cts.70484","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T15:51:58Z","timestamp":1769529118000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Pacific Blue Derivatives of Paclitaxel as Fluorescent Probes of OATP1B\u2010Type Transporters"],"prefix":"10.1111","volume":"19","author":[{"given":"Mahesh R.","family":"Nepal","sequence":"first","affiliation":[{"name":"Division of Pharmaceutics and Pharmacology The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2755-4719","authenticated-orcid":false,"given":"Yue","family":"Xu","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutics and Pharmacology The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Jin","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutics and Pharmacology The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Hu","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutics and Pharmacology The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojun","family":"Hu","sequence":"additional","affiliation":[{"name":"Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Digamber S.","family":"Rane","sequence":"additional","affiliation":[{"name":"Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo E.","family":"Andres","sequence":"additional","affiliation":[{"name":"Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman A.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutics and Pharmacology The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brandon","family":"Steiger","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutics and Pharmacology The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2660-6644","authenticated-orcid":false,"given":"Alex","family":"Sparreboom","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutics and Pharmacology The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blake R.","family":"Peterson","sequence":"additional","affiliation":[{"name":"Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9806-7734","authenticated-orcid":false,"given":"Shuiying","family":"Hu","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutics and Pharmacology The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2015.04.011"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2014.10.014"},{"key":"e_1_2_9_4_1","first-page":"389","article-title":"Distribution of Paclitaxel Within the Nervous System of the Rat After Repeated Intravenous Administration","volume":"21","author":"Cavaletti G.","year":"2000","journal-title":"Neurotoxicology"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI96160"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-13-3634"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1983-4_28"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201703298"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomedchemau.2c00031"},{"key":"e_1_2_9_10_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0237096","article-title":"RandoMice, a Novel, User\u2010Friendly Randomization Tool in Animal Research","volume":"15","author":"Eenige R.","year":"2020","journal-title":"PLoS 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