{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T15:30:06Z","timestamp":1789399806281,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:00:00Z","timestamp":1769644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:00:00Z","timestamp":1769644800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["ascpt.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Translational Sci"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Severe thalassemia remains a significant public health concern in Southeast Asia. Prenatal screening is an effective strategy for early detection and prevention. This study aimed to determine the prevalence of severe thalassemia and assess the performance of prenatal screening at the Siriraj Thalassemia Center, Siriraj Hospital, Thailand. A retrospective review was conducted using data from January 2018 to December 2023. A total of 18,976 pregnant women underwent initial screening with mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and hemoglobin (Hb) typing. Of these, 12,499 tested positive. Complete screening data from 6,698 male partners identified 18.3% of couples as high\u2010risk. Deoxyribonucleic acid (DNA) analysis and prenatal diagnostic testing were performed for at\u2010risk pregnancies. Among high\u2010risk couples, 75.2% of fetuses were identified by DNA analysis as being at risk for severe thalassemia, and 34.2% were confirmed as affected. The distribution of severe thalassemia types included Hb Bart's Hydrops Fetalis (15.8%), homozygous \u03b2\u2010thalassemia (1.6%), and \u03b2\u2010thalassemia\/Hb E disease (16.8%). The most frequent \u03b1\u2010thalassemia genotype in Hb Bart's cases was homozygous \u03b1\n                    <jats:sup>0<\/jats:sup>\n                    \u2010thalassemia (\u2010\u2010\n                    <jats:sup>SEA<\/jats:sup>\n                    \/\u2010\u2010\n                    <jats:sup>SEA<\/jats:sup>\n                    ; 96.3%). The most common \u03b2\u2010thalassemia mutation was a 4\u2010base pair deletion at codons 41\/42 (\u2010TTCT; 40.2%). DNA testing for \u03b1\u2010thalassemia showed 100% specificity and positive predictive value (PPV). For \u03b2\u2010thalassemia, the sensitivity, specificity, PPV, and negative predictive value (NPV) were 97.6%, 98.9%, 96.1%, and 99.3%, respectively. The findings underscore the effectiveness of prenatal screening and diagnosis in identifying severe thalassemia, highlighting their importance for informing prevention strategies and guiding public health planning in high\u2010prevalence settings.\n                  <\/jats:p>","DOI":"10.1111\/cts.70485","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T07:01:07Z","timestamp":1769756467000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Prevalence of Severe Thalassemia and Performance of Prenatal Screening Tests Among Pregnant Women at Siriraj Thalassemia Center in Thailand"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-4204-467X","authenticated-orcid":false,"given":"Kwandao","family":"Malasai","sequence":"first","affiliation":[{"name":"Social, Economic and Administrative Pharmacy (SEAP) Graduate Program, Faculty of Pharmacy Mahidol University  Bangkok Thailand"},{"name":"Social Pharmacy Research Unit, Faculty of Pharmacy Mahasarakham University  Maha Sarakham Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9457-9823","authenticated-orcid":false,"given":"Usa","family":"Chaikledkaew","sequence":"additional","affiliation":[{"name":"Social and Administrative Pharmacy Division, Department of Pharmacy, Faculty of Pharmacy Mahidol University  Bangkok Thailand"},{"name":"Mahidol University Health Technology Assessment (MUHTA) Graduate Program Mahidol University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3668-8754","authenticated-orcid":false,"given":"Pattarawalai","family":"Talungchit","sequence":"additional","affiliation":[{"name":"Mahidol University Health Technology Assessment (MUHTA) Graduate Program Mahidol University  Bangkok Thailand"},{"name":"Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital Mahidol University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2448-3954","authenticated-orcid":false,"given":"Sitaporn","family":"Youngkong","sequence":"additional","affiliation":[{"name":"Social and Administrative Pharmacy Division, Department of Pharmacy, Faculty of Pharmacy Mahidol University  Bangkok Thailand"},{"name":"Mahidol University Health Technology Assessment (MUHTA) Graduate Program Mahidol University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1132-7442","authenticated-orcid":false,"given":"Wanvisa","family":"Udomsinprasert","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Mahidol University  Bangkok Thailand"},{"name":"Biomarker and Epigenetic Science for Targeted Therapeutics (BEST) Research Center Faculty of Pharmacy, Mahidol University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7374-6765","authenticated-orcid":false,"given":"Chanin","family":"Limwongse","sequence":"additional","affiliation":[{"name":"Division of Medical Genetics, Department of Medicine, Faculty of Medicine Siriraj Hospital Mahidol University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4987-3497","authenticated-orcid":false,"given":"Jiraphun","family":"Jittikoon","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Mahidol University  Bangkok Thailand"},{"name":"Biomarker and Epigenetic Science for Targeted Therapeutics (BEST) Research Center Faculty of Pharmacy, Mahidol University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,29]]},"reference":[{"issue":"4","key":"e_1_2_9_2_1","first-page":"498","article-title":"Haemoglobinopathies in Southeast Asia","volume":"134","author":"Fucharoen S.","year":"2011","journal-title":"Indian Journal of Medical Research"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/03630269.2022.2025824"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1990.tb24288.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.2514"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1345.004"},{"key":"e_1_2_9_7_1","unstructured":"Thai Society of Hematology \u201cGuidelines for the Management of Thalassemia \u201d2019 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