{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T17:35:44Z","timestamp":1788975344260,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["ascpt.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Translational Sci"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This study aimed to evaluate the diagnostic utility of urinary exosomal microRNAs (miRNAs) in subclinical rejection following kidney transplantation by comparing miRNA expression profiles in urinary exosomes between patients with no evidence of rejection and patients with subclinical T\u2010cell\u2010mediated rejection (sc\u2010TCMR), as confirmed by protocol kidney biopsies performed 3\u2009months after transplantation. To elucidate these differences, a comprehensive miRNA expression analysis was conducted using microarray profiling. Consequently, 38 urinary exosomal miRNAs were detected, among which three were upregulated and five were downregulated in patients with sc\u2010TCMR. To further evaluate the diagnostic value of these miRNAs, quantitative real\u2010time PCR analysis was performed using urinary exosomes collected at the time of protocol biopsy from 70 kidney transplant recipients. This analysis confirmed that miR\u20105100 and miR\u20107975 were differentially expressed in patients with sc\u2010TCMR at the time of the 12\u2010month protocol biopsy. Importantly, miR\u20107975 demonstrated the ability to distinguish among three groups\u2014no evidence of rejection, borderline changes, and sc\u2010TCMR\u2014with high diagnostic accuracy, as indicated by an area under the receiver operating characteristic curve of 0.825. In\u00a0vitro, exposure of proximal tubular epithelial cells to transforming growth factor\u2010beta 1 resulted in a reduction in miR\u20107975 expression within urinary exosomes, implicating these cells as a potential source of exosomal miR\u20107975. Collectively, these findings suggest that urinary exosomal miR\u20107975 may serve as a promising noninvasive biomarker for diagnosing and monitoring sc\u2010TCMR, offering valuable insights for future research and clinical applications.<\/jats:p>","DOI":"10.1111\/cts.70572","type":"journal-article","created":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T03:19:46Z","timestamp":1777691986000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification of Urinary Exosomal\n                    <scp>microRNAs<\/scp>\n                    Associated With Subclinical Acute T\u2010Cell\u2010Mediated Rejection in Kidney Transplant Recipients"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-4679-3356","authenticated-orcid":false,"given":"Soichiro","family":"Tajima","sequence":"first","affiliation":[{"name":"Department of Pharmacy Kyushu University Hospital  Fukuoka 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