{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T05:47:10Z","timestamp":1782107230138,"version":"3.54.5"},"reference-count":44,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100012774","name":"Innovationsfonden","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012774","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001734","name":"K\u00f8benhavns Universitet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001734","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100023331","name":"LEO Pharma","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100023331","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ascpt.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Translational Sci"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Valid pre\u2010clinical animal models are essential for effective drug development in inflammatory skin diseases like atopic dermatitis. Most murine models exhibit low\u2010to\u2010moderate inflammatory responses with significant variability, undermining their validity and contributing to high drug attrition rates. Previous studies showed that microbiota transfer and early\u2010life microbial exposure affect inflammatory phenotypes and allergy development. This concept might extend to other pre\u2010clinical inflammatory models. We tested the impact of different microbiotas on ovalbumin (OVA)\u2010induced skin inflammation in mice, by using BALB\/c substrains from different vendors. Additionally, germ\u2010free mice were co\u2010housed for 2\u2009weeks with specific\u2010pathogen\u2010free (SPF) mice at 6\u2009weeks of age, resulting in delayed gut colonization. Thereafter, mice were sensitized with OVA in aluminum hydroxide and challenged with OVA on the ear. Skin inflammation was assessed through ear cytokine and serum immunoglobulin E (IgE) levels. Different T cell populations were analyzed in the spleen and ear\u2010draining lymph node with flow cytometry. Microbiota composition was analyzed via 16S rRNA sequencing. Despite substantial differences in microbiota composition as well as T cell proportion across vendors, similar inflammatory responses to OVA were observed. An early sterile environment resulted in elevated serum IgE and fewer T cells in ear\u2010draining lymph nodes compared to SPF mice but did not affect ear tissue cytokines. These studies demonstrate that the OVA\u2010induced skin inflammation model is robust to microbiota differences, enhancing reproducibility while highlighting persistent issues of variability and low effect size.<\/jats:p>","DOI":"10.1111\/cts.70593","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T08:22:11Z","timestamp":1780388531000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Inflammatory Response in the Murine Ovalbumin\u2010Induced Skin Inflammation Model Is Robust Despite Major Gut Microbiota Differences"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1583-8749","authenticated-orcid":false,"given":"Ida Wang","family":"Henriksen","sequence":"first","affiliation":[{"name":"Section of Biomedicine, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences University of Copenhagen  Frederiksberg C Denmark"},{"name":"Skin Sciences, Search, Science &amp; Innovation LEO Pharma A\/S  Ballerup Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4422-9133","authenticated-orcid":false,"given":"Christina","family":"Bartholdy","sequence":"additional","affiliation":[{"name":"Skin Sciences, Search, Science &amp; Innovation LEO Pharma A\/S  Ballerup Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janne","family":"Koch","sequence":"additional","affiliation":[{"name":"In Vivo Cell Efficacy and Histology Novo Nordisk A\/S  M\u00e5l\u00f8v Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4366-3827","authenticated-orcid":false,"given":"Denitsa Vladimirova","family":"Stefanova","sequence":"additional","affiliation":[{"name":"Department of Food Science University of Copenhagen  Frederiksberg C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1860-385X","authenticated-orcid":false,"given":"Camilla Hartmann Friis","family":"Hansen","sequence":"additional","affiliation":[{"name":"Section of Biomedicine, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences University of Copenhagen  Frederiksberg C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1575-2507","authenticated-orcid":false,"given":"Axel Kornerup","family":"Hansen","sequence":"additional","affiliation":[{"name":"Section of Biomedicine, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences University of Copenhagen  Frederiksberg C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000370220"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882\u2010019\u20100893\u2010z"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2012-0500OC"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/cts.13697"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.4172\/2155\u20109899.1000110"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1222878110"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.5.2731"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1700453"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0153151"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1312"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms231911532"},{"key":"e_1_2_10_13_1","unstructured":"EU Commission \u201cSummary Report on the Statistics on the Use of Animals for Scientific Purposes in the Member States of the European Union and Norway in 2022 \u201d EU Commission (2024) https:\/\/www.understandinganimalresearch.org.uk\/news\/eu\u2010wide\u2010animal\u2010research\u2010statistics\u20102022."},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2021.1993581"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2019.02.014"},{"key":"e_1_2_10_16_1","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.01504-23","article-title":"Spontaneous Episodic Inflammation in the Intestines of Mice Lacking HNF4A Is Driven by Microbiota and Associated With Early Life Microbiota Alterations","volume":"14","author":"Kelly C.","year":"2023","journal-title":"MBio"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2016.04.003"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaw4361"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/cts.13697Digital"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature17655"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199606273342603"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa063842"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00228-005-0060-4"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00335-021-09866-4"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/v11050435"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201017242\u20108"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-70765-w"},{"key":"e_1_2_10_28_1","first-page":"401","article-title":"Variation in Airway Responsiveness of Male C57BL\/6 Mice From 5 Vendors","volume":"51","author":"Chang H.\u2010Y. 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