{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T14:24:08Z","timestamp":1787408648006,"version":"build-2736575974"},"reference-count":28,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["ascpt.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Translational Sci"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Chagas disease is a neglected tropical disease endemic in most of the Americas, caused by\n                    <jats:italic>Trypanosoma cruzi<\/jats:italic>\n                    . New serological tests with improved performance are urgently needed for accurate diagnosis of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>T. cruzi<\/jats:italic>\n                    <\/jats:styled-content>\n                    infections. We evaluated here the performance of a novel peptide ELISA based on highly conserved parasite antigens, using a large panel of well characterized samples (\n                    <jats:italic>N<\/jats:italic>\n                    \u2009=\u2009308) from Mexico, where serological discordance is particularly high. Comparison of peptide ELISA with various serological and molecular assays indicated that it outperformed an FDA\u2010approved commercial ELISA in terms of sensitivity (47.5% vs. 28.3% compared with PCR), while a high specificity was maintained (80.3%\u201391.2% compared with PCR or serology plus PCR). Improved sensitivity was due to the reactivity of samples previously characterized as serodiscordants. Nonetheless, test performance remains limited for stand\u2010alone use but may complement current tests. Further optimizing antigen composition to include additional highly conserved antigens may allow reaching optimum levels of test performance to simplify serological diagnosis of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>T. cruzi<\/jats:italic>\n                    <\/jats:styled-content>\n                    infection.\n                  <\/jats:p>","DOI":"10.1111\/cts.70676","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T12:40:03Z","timestamp":1784896803000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of a Novel Peptide\u2010Based\n                    <scp>ELISA<\/scp>\n                    for the Serological Diagnosis of\n                    <i>Trypanosoma cruzi<\/i>\n                    Infection"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-7356-8654","authenticated-orcid":false,"given":"Alicia","family":"Majeau","sequence":"first","affiliation":[{"name":"Department of Tropical Medicine and Infectious Disease Celia Scott Weatherhead School of Public Health and Tropical Medicine, Tulane University  New Orleans USA"},{"name":"Laboratorio de Parasitolog\u00eda, Centro de Investigaciones Regionales \u201cDr Hideyo Noguchi\u201d Universidad Aut\u00f3noma de Yucat\u00e1n  M\u00e9rida Yucat\u00e1n M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4931-146X","authenticated-orcid":false,"given":"Julio Vladimir","family":"Cruz\u2010Chan","sequence":"additional","affiliation":[{"name":"Laboratorio de Parasitolog\u00eda, Centro de Investigaciones Regionales \u201cDr Hideyo Noguchi\u201d Universidad Aut\u00f3noma de Yucat\u00e1n  M\u00e9rida Yucat\u00e1n M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3270-6476","authenticated-orcid":false,"given":"Etienne","family":"Waleckx","sequence":"additional","affiliation":[{"name":"Laboratorio de Parasitolog\u00eda, Centro de Investigaciones Regionales \u201cDr Hideyo Noguchi\u201d Universidad Aut\u00f3noma de Yucat\u00e1n  M\u00e9rida Yucat\u00e1n M\u00e9xico"},{"name":"INTERTRYP, Universit\u00e9 de Montpellier, Cirad, IRD  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9376-0209","authenticated-orcid":false,"given":"Eric","family":"Dumonteil","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine and Infectious Disease Celia Scott Weatherhead School of Public Health and Tropical Medicine, Tulane University  New Orleans USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9009-4201","authenticated-orcid":false,"given":"Claudia","family":"Herrera","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine and Infectious Disease Celia Scott Weatherhead School of Public Health and Tropical Medicine, Tulane University  New Orleans USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2009.11.003"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(25)00562-6"},{"key":"e_1_2_8_4_1","unstructured":"PAHO \u201cGuidelines for the Diagnosis and Treatment of Chagas Disease \u201d176 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