{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:52:10Z","timestamp":1792173130496,"version":"4.4.0"},"reference-count":16,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["ascpt.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Translational Sci"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>BK polyomavirus (BKPyV) infection may lead to BKPyV\u2010associated nephropathy (BKPyVAN) and subsequent graft loss in kidney transplant recipients. Experimental studies have suggested that glucocorticoids (GCs) may enhance BKPyV replication through a steroid\u2010responsive element within the viral genome. However, clinical observations have not consistently supported this association, prompting re\u2010investigation of the effect of GCs on BKPyV replication in primary human renal epithelial cells (PTECs). PTECs were infected with BKPyV and subsequently exposed to increasing concentrations of methylprednisolone (MPS) or dexamethasone (Dexa). Viral replication was quantified by quantitative polymerase chain reaction (qPCR). Relative differences in viral load were analyzed using univariate analysis of variance. Activation of the GC pathway was assessed by measuring FKBP5 and PER1 gene expression. Inflammatory responses were evaluated by enzyme\u2010linked immunosorbent assays (ELISA) for IL\u20106, IL\u20108, and TNF\u2010\u03b1. Treatment of BKPyV\u2010infected PTECs with increasing concentrations of MPS or Dexa did not affect viral replication compared to untreated controls. While treatment\u2010related changes in gene expression of FKBP5 and PER1 were observed, confirming GC\u2010signaling pathway activation in BKPyV\u2010infected cells. Cytokine production (IL\u20106 and IL\u20108) was unaffected. In primary human PTECs infected with BKPyV, treatment with MPS or Dexa did not enhance BKPyV replication. These findings contrast with earlier in\u00a0vitro studies and suggest that glucocorticoids do not directly promote BKPyV replication under the conditions tested. Further studies are needed to determine whether these findings extend to other cell models and in\u00a0vivo.<\/jats:p>","DOI":"10.1111\/cts.70736","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:38:02Z","timestamp":1790836682000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Glucocorticoids Do Not Enhance\n                    <scp>BK<\/scp>\n                    Polyomavirus Replication in Human Renal Proximal Tubular Epithelial Cells In\u00a0Vitro"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7442-5701","authenticated-orcid":false,"given":"Wouter T.","family":"Moest","sequence":"first","affiliation":[{"name":"Department of Internal Medicine Leiden University Medical Center (LUMC)  Leiden the Netherlands"},{"name":"Leiden Transplant Center Leiden University Medical Center (LUMC)  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reshma A.","family":"Lalai","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Leiden University Medical Center (LUMC)  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6499-5076","authenticated-orcid":false,"given":"Jurri\u00ebn","family":"Prins","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Leiden University Medical Center (LUMC)  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9284-3595","authenticated-orcid":false,"given":"Aiko P. J.","family":"de Vries","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Leiden University Medical Center (LUMC)  Leiden the Netherlands"},{"name":"Leiden Transplant Center Leiden University Medical Center (LUMC)  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6707-2311","authenticated-orcid":false,"given":"Els","family":"Wessels","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology &amp; Infection Prevention Leiden University Medical Center (LUMC)  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6257-0899","authenticated-orcid":false,"given":"Cees","family":"van Kooten","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Leiden University Medical Center (LUMC)  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5993-9846","authenticated-orcid":false,"given":"Mariet C. W.","family":"Feltkamp","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology &amp; Infection Prevention Leiden University Medical Center (LUMC)  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9682-6234","authenticated-orcid":false,"given":"Joris I.","family":"Rotmans","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Leiden University Medical Center (LUMC)  Leiden the Netherlands"},{"name":"Leiden Transplant Center Leiden University Medical Center (LUMC)  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000004976"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/ctr.13528"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.70084"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.5500\/wjt.v5.i4.292"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa020439"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.4.2398-2408.1994"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/147.4.676"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.5858\/2005-129-69-KAUTPI"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00238-15"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.13880"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M209836200"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000001521"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.00343-23"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-022-01962-0"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1992.299"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/ti.2025.15122"}],"container-title":["Clinical and Translational Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/ascpt.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cts.70736","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ascpt.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cts.70736","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ascpt.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cts.70736","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:17:45Z","timestamp":1792171065000},"score":1,"resource":{"primary":{"URL":"https:\/\/ascpt.onlinelibrary.wiley.com\/doi\/10.1111\/cts.70736"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":16,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/cts.70736"],"URL":"https:\/\/doi.org\/10.1111\/cts.70736","archive":["Portico"],"relation":{},"ISSN":["1752-8054","1752-8062"],"issn-type":[{"value":"1752-8054","type":"print"},{"value":"1752-8062","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]},"assertion":[{"value":"2026-05-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70736"}}