{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T02:45:18Z","timestamp":1789094718224,"version":"build-2803163510"},"reference-count":14,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,2,11]],"date-time":"2013-02-11T00:00:00Z","timestamp":1360540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Fluorescence of pathogenic fungi has been previously shown when hematoxylin and eosin\u2010stained sections are examined under a fluorescent microscope. We hypothesize that this phenomenon could aid in the evaluation of nail specimens for onychomycosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Forty\u2010eight routinely stained nail sections of periodic acid\u2010Schiff (<jats:styled-content style=\"fixed-case\">PAS<\/jats:styled-content>)\u2010positive onychomycosis, along with 23 <jats:styled-content style=\"fixed-case\">PAS<\/jats:styled-content>\u2010negative control specimens with a clinical diagnosis of onychomycosis, were studied under a fluorescent microscope to determine the clinical usefulness of this technique.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In most cases, fluorescence of fungal organisms was noted. Fungi were identified by their tubular or annular shapes with fluorescence surrounding them. The sensitivity and specificity of the method were 96 and 90%, respectively. In some cases, it was difficult to identify the fungi because of the relative paucity of organisms, weak fluorescence and high background fluorescence of eosinophilic nail keratin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We conclude that fluorescence microscopy can be used as a rapid screening tool for identification of fungi in nail specimens.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.12086","type":"journal-article","created":{"date-parts":[[2013,1,10]],"date-time":"2013-01-10T17:55:34Z","timestamp":1357840534000},"page":"385-390","source":"Crossref","is-referenced-by-count":29,"title":["The diagnostic value of fungal fluorescence in onychomycosis"],"prefix":"10.1111","volume":"40","author":[{"given":"Munir H.","family":"Idriss","sequence":"first","affiliation":[{"name":"Ackerman Academy of Dermatology  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Khalil","sequence":"additional","affiliation":[{"name":"Department of Pathology State University of New York Downstate Medical Center  Brooklyn NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Elston","sequence":"additional","affiliation":[{"name":"Ackerman Academy of Dermatology  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,2,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1001\/jama.1967.03130240077014","article-title":"Medical uses of the Wood's lamp","volume":"202","author":"Caplan RM","year":"1967","journal-title":"JAMA"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/79.5.587"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/79.2.231"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0039-6257(85)90114-6"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1067\/mjd.2002.120623"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.2009.01468.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2006.07493.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2010.03704.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"crossref","first-page":"258","DOI":"10.7547\/0950258","article-title":"Utility of histopathologic analysis in the evaluation of onychomycosis","volume":"95","author":"Weinberg JM","journal-title":"J Am Podiatr Med Assoc"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.2007.00890.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-1-21"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0b013e318234cc49"},{"key":"e_1_2_6_14_1","first-page":"93","article-title":"Autofluorescence of eosinophilic substances","volume":"110","author":"Huntington H","year":"1986","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_6_15_1","first-page":"215","article-title":"Autofluorescence: a screening test for mycotic infection in tissues","volume":"51","author":"Rao S","journal-title":"Indian J Pathol Microbiol"}],"container-title":["Journal of Cutaneous Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcup.12086","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cup.12086","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T17:32:44Z","timestamp":1700674364000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cup.12086"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,2,11]]},"references-count":14,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,4]]}},"alternative-id":["10.1111\/cup.12086"],"URL":"https:\/\/doi.org\/10.1111\/cup.12086","archive":["Portico"],"relation":{},"ISSN":["0303-6987","1600-0560"],"issn-type":[{"value":"0303-6987","type":"print"},{"value":"1600-0560","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,2,11]]}}}