{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T22:11:22Z","timestamp":1773785482016,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,4,2]],"date-time":"2014-04-02T00:00:00Z","timestamp":1396396800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2014,6]]},"abstract":"CD31<\/jats:styled-content> is a standard immunostain for evaluating vascular lesions of the skin, but CD31<\/jats:styled-content> reactivity for histiocytes is reported in only a small variety of pathological conditions. CD68<\/jats:styled-content> and CD163<\/jats:styled-content> are well recognized stains for cutaneous histiocytic lesions. We compared immunostaining of CD31<\/jats:styled-content> within that of CD68<\/jats:styled-content> and CD163<\/jats:styled-content> in five cases each of cutaneous lesions containing histiocytes: healing biopsy site, granuloma annulare, xanthogranuloma, ruptured follicular cyst and sarcoidosis. Reactivity was graded on a scale of 0\u20133 for brightness of immunostaining. Immunoreactivity was seen in histiocytes in all specimens for CD31<\/jats:styled-content>, CD68<\/jats:styled-content> and CD163<\/jats:styled-content>. The average intensity of staining was 1.7\u20132.5 for CD31<\/jats:styled-content>, 2.6\u20133 for CD68<\/jats:styled-content> and 2.9\u20133 for CD163<\/jats:styled-content>. The staining was somewhat less for CD31<\/jats:styled-content> because the reactivity is localized on the cell surfaces, whereas CD68<\/jats:styled-content> and CD163<\/jats:styled-content> react with cell surfaces and cytoplasm. We conclude that histiocytes in cutaneous lesions stain for CD31<\/jats:styled-content> and the staining is comparable to, but less intense, than that seen with CD68<\/jats:styled-content> and CD163<\/jats:styled-content>. Caution is suggested in interpretation of CD31<\/jats:styled-content> staining in skin specimens, as CD31<\/jats:styled-content> shows reactivity with histiocytes as well as endothelial cells.<\/jats:p>","DOI":"10.1111\/cup.12327","type":"journal-article","created":{"date-parts":[[2014,3,10]],"date-time":"2014-03-10T14:05:47Z","timestamp":1394460347000},"page":"489-493","source":"Crossref","is-referenced-by-count":11,"title":["Tissue histiocyte reactivity with CD31<\/scp> is comparable to CD68<\/scp> and CD163<\/scp> in common skin lesions"],"prefix":"10.1111","volume":"41","author":[{"given":"W. James","family":"Tidwell","sequence":"first","affiliation":[{"name":"Department of Medicine University of Tennessee Graduate School of Medicine Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul B.","family":"Googe","sequence":"additional","affiliation":[{"name":"Knoxville Dermatopathology Laboratory Knoxville TN USA"},{"name":"Department of Pathology, University of Tennessee Graduate School of Medicine Knoxville TN USA"},{"name":"Department of Pathology, Vanderbilt University Nashville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,4,2]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"PECAM1 platelet\/endothelial cell adhesion molecule 1(2013). 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(2013). Available at:www.ncbi.nlm.nih.gov\/gene\/968[accessed on 20 December 2013]."},{"key":"e_1_2_6_18_1","unstructured":"Leica Biosystems. (2013).CD163 primary antibodies. Available at:http:\/\/www.leicabiosystems.com\/ihc-ish\/novocastra-reagents\/primary-antibodies\/details\/product\/cd163\/[accessed on 20 December 2013]."},{"key":"e_1_2_6_19_1","unstructured":"CD163 Molecule. (2013). 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