{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T04:25:38Z","timestamp":1771302338642,"version":"3.50.1"},"reference-count":5,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2014,11,3]],"date-time":"2014-11-03T00:00:00Z","timestamp":1414972800000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Evaluation of a potential immunobullous disorder typically requires two pieces of tissue obtained by skin biopsy: one placed in formalin for conventional microscopy and a second placed in a different transport medium suitable for direct immunofluorescence (<jats:styled-content style=\"fixed-case\">DIF<\/jats:styled-content>) testing. Clinical practice in this area is not standardized, with dermatologists either obtaining two biopsies or dividing (pre\u2010bisecting) a single biopsy. Some <jats:styled-content style=\"fixed-case\">DIF<\/jats:styled-content> specimens are technically inadequate for interpretation of subepidermal imunobullous disorders because the basement membrane zone is not intact, but it is unknown whether pre\u2010bisecting the tissue increases the risk of compromising the specimen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate whether technically inadequate <jats:styled-content style=\"fixed-case\">DIF<\/jats:styled-content> specimens are associated with pre\u2010bisection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">DIF<\/jats:styled-content> specimens were consecutively sampled from a single referral center and identified as whole (non\u2010bisected) biopsy specimens or pre\u2010bisected biopsy specimens. The proportion of inadequate specimens was calculated for both groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 3450 specimens were included. The percentage of inadequate specimens was 5.072% (153\/3016) for whole (non\u2010bisected) specimens and 5.299% for pre\u2010bisected specimens. This difference was not significant (chi square, p = 0.84). The study was sufficiently powered to detect a relative risk of 1.685.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Pre\u2010bisection of a single skin biopsy does not significantly increase the risk of a technically inadequate specimen for direct immunofluorescence testing.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.12393","type":"journal-article","created":{"date-parts":[[2014,9,26]],"date-time":"2014-09-26T18:26:59Z","timestamp":1411756019000},"page":"890-892","source":"Crossref","is-referenced-by-count":3,"title":["Pre\u2010bisection of a single skin biopsy does not produce technically inadequate specimens for direct immunofluorescence: a review of 3450 specimens"],"prefix":"10.1111","volume":"41","author":[{"given":"Ern","family":"Loh","sequence":"first","affiliation":[{"name":"Department of Dermatology University of California Davis School of Medicine  Sacramento CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"April W.","family":"Armstrong","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Colorado Denver School of Medicine  Aurora CO USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maxwell A.","family":"Fung","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of California Davis School of Medicine  Sacramento CA USA"},{"name":"Department of Pathology University of California Davis School of Medicine  Sacramento CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,11,3]]},"reference":[{"key":"e_1_2_5_2_1","unstructured":"Qu Z Ghorbani R Anders\u2010DiPonio M Le M. Skin: punch biopsy. 2006\u20132012. URLwww.essentialpathology.info\/Gross_manual\/SkinPunchBiopsy.html[accessed on 10 October 2014]."},{"key":"e_1_2_5_3_1","volume-title":"Dermatologic surgery tips and techniques","author":"Salasche S","year":"2007"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2010.06.019"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12198"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-5945-4-10"}],"container-title":["Journal of Cutaneous Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcup.12393","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cup.12393","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T03:32:46Z","timestamp":1696217566000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cup.12393"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11]]},"references-count":5,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2014,11]]}},"alternative-id":["10.1111\/cup.12393"],"URL":"https:\/\/doi.org\/10.1111\/cup.12393","archive":["Portico"],"relation":{},"ISSN":["0303-6987","1600-0560"],"issn-type":[{"value":"0303-6987","type":"print"},{"value":"1600-0560","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,11]]}}}