{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T13:51:43Z","timestamp":1785678703692,"version":"3.56.0"},"reference-count":14,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,12,15]],"date-time":"2014-12-15T00:00:00Z","timestamp":1418601600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["1R43CA168019-11"],"award-info":[{"award-number":["1R43CA168019-11"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2015,2]]},"abstract":"\n Background<\/jats:title>\n \n Detection of immunoglobulin light\u2010chain restriction is important in the diagnosis of B\u2010cell non\u2010Hodgkin lymphoma (\n B\u2010NHL<\/jats:styled-content>\n ). Flow\u2010cytometry, commonly used to evaluate light\u2010chain restriction, is impractical to be used in cutaneous specimens. Immunohistochemical and conventional chromogenic\n in situ<\/jats:italic>\n hybridization (\n CISH<\/jats:styled-content>\n ) methods on formalin\u2010fixed\u2010paraffin\u2010embedded (\n FFPE<\/jats:styled-content>\n ) tissue lack sufficient sensitivity to detect low\u2010level light\u2010chain expression in B\u2010\n NHL<\/jats:styled-content>\n without plasmacytic differentiation. Ultrasensitive bright\u2010field\n mRNA\u2010ISH<\/jats:styled-content>\n (\n BRISH<\/jats:styled-content>\n ) for\n in situ<\/jats:italic>\n light\u2010chain detection in cutaneous B\u2010\n NHL<\/jats:styled-content>\n has been assessed.\n <\/jats:p>\n <\/jats:sec>\n \n Design<\/jats:title>\n \n Kappa\/lambda\n mRNA<\/jats:styled-content>\n was detected using two\u2010color\n BRISH<\/jats:styled-content>\n (RNAscope 2xPlex, Advanced Cell Diagnostics) on 27\n FFPE<\/jats:styled-content>\n skin biopsies and excisions from patients with available B\u2010cell\n PCR<\/jats:styled-content>\n clonality studies: 16 clonal B\u2010cell lesions (6 follicle center lymphoma, 5 marginal zone lymphoma, 3 large B\u2010cell lymphoma, and 2 other) and 11 non\u2010clonal B\u2010cell proliferations.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n BRISH<\/jats:styled-content>\n was successful in 15\/16 clonal B\u2010cell lesions and 11\/11 non\u2010clonal proliferations. Light\u2010chain restriction was detected in 15\/15 clonal lesions and in 1\/11 non\u2010clonal proliferations (96.1% overall concordance with clonality\n PCR<\/jats:styled-content>\n ). In 4\/5 marginal zone lymphomas, light\u2010chain restriction was detected as strong monotypic\n mRNA<\/jats:styled-content>\n expression in a B\u2010cell subset, consistent with plasmacytic differentiation.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Ultrasensitive\n BRISH<\/jats:styled-content>\n can successfully detect light\u2010chain restriction in B\u2010\n NHL<\/jats:styled-content>\n from\n FFPE<\/jats:styled-content>\n skin specimens and may be a useful adjunct ancillary tool in cases not resolved by\n CISH<\/jats:styled-content>\n or immunohistochemical methods.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/cup.12415","type":"journal-article","created":{"date-parts":[[2014,11,3]],"date-time":"2014-11-03T04:06:12Z","timestamp":1414987572000},"page":"82-89","source":"Crossref","is-referenced-by-count":16,"title":["Detection of immunoglobulin light\u2010chain restriction in cutaneous B\u2010cell lymphomas by ultrasensitive bright\u2010field\n mRNA<\/scp>\n in situ<\/i>\n hybridization"],"prefix":"10.1111","volume":"42","author":[{"given":"Eugen C","family":"Minca","sequence":"first","affiliation":[{"name":"Department of Pathology Cleveland Clinic Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongwei","family":"Wang","sequence":"additional","affiliation":[{"name":"Advanced Cell Diagnostics Hayward CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology Cleveland Clinic Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Lanigan","sequence":"additional","affiliation":[{"name":"Department of Pathology Cleveland Clinic Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven D","family":"Billings","sequence":"additional","affiliation":[{"name":"Department of Pathology Cleveland Clinic Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuling","family":"Luo","sequence":"additional","affiliation":[{"name":"Advanced Cell Diagnostics Hayward CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond R","family":"Tubbs","sequence":"additional","affiliation":[{"name":"Department of Pathology Cleveland Clinic Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Jun","family":"Ma","sequence":"additional","affiliation":[{"name":"Advanced Cell Diagnostics Hayward CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,12,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-10-184168"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-09-3502"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.11.7096"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1755-8794-4-27"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403202"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-171-4_12"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2008.08670.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-11-120535"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/00019606-199409000-00006"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPJTWK07FSABRJ"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-1459-3_12"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00019606-200303000-00002"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sder.2012.08.006"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2012.181"}],"container-title":["Journal 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