{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:00:52Z","timestamp":1792119652946,"version":"4.4.0"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,4,13]],"date-time":"2015-04-13T00:00:00Z","timestamp":1428883200000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Myriad Genetic Laboratories, Inc."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Histopathologic examination is sometimes inadequate for accurate and reproducible diagnosis of certain melanocytic neoplasms. As a result, more sophisticated and objective methods have been sought. The goal of this study was to identify a gene expression signature that reliably differentiated benign and malignant melanocytic lesions and evaluate its potential clinical applicability. Herein, we describe the development of a gene expression signature and its clinical validation using multiple independent cohorts of melanocytic lesions representing a broad spectrum of histopathologic subtypes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Using quantitative reverse\u2010transcription polymerase chain reaction (\n                      <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                      ) on a selected set of 23 differentially expressed genes, and by applying a threshold value and weighting algorithm, we developed a gene expression signature that produced a score that differentiated benign nevi from malignant melanomas.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The gene expression signature classified melanocytic lesions as benign or malignant with a sensitivity of 89% and a specificity of 93% in a training cohort of 464 samples. The signature was validated in an independent clinical cohort of 437 samples, with a sensitivity of 90% and specificity of 91%.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The performance, objectivity, reliability and minimal tissue requirements of this test suggest that it could have clinical application as an adjunct to histopathology in the diagnosis of melanocytic neoplasms.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cup.12475","type":"journal-article","created":{"date-parts":[[2015,3,2]],"date-time":"2015-03-02T05:11:03Z","timestamp":1425273063000},"page":"244-252","source":"Crossref","is-referenced-by-count":139,"title":["Clinical validation of a gene expression signature that differentiates benign nevi from malignant melanoma"],"prefix":"10.1111","volume":"42","author":[{"given":"Loren E.","family":"Clarke","sequence":"first","affiliation":[{"name":"Myriad Genetic Laboratories, Inc.  Salt Lake City UT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.B.","family":"Warf","sequence":"additional","affiliation":[{"name":"Myriad Genetic Laboratories, Inc.  Salt Lake City UT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"II","given":"Darl D.","family":"Flake","sequence":"additional","affiliation":[{"name":"Myriad Genetics, Inc.  Salt Lake City UT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne\u2010Renee","family":"Hartman","sequence":"additional","affiliation":[{"name":"Myriad Genetic Laboratories, Inc.  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