{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T20:52:25Z","timestamp":1760043145918},"reference-count":21,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,6,8]],"date-time":"2015-06-08T00:00:00Z","timestamp":1433721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>p16 immunostaining has been used to aid and improve the histopathologic evaluation of equivocal cervical lesions with associated low\u2010grade or high\u2010grade dysplasia. However, the utility of p16 immunostaining in the diagnosis of atypical genital skin lesions remains debatable.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We conducted a cross\u2010sectional study of genital skin lesions with varying degrees of atypia. Four pathologists assessed lesional atypia and interpreted hematoxylin and eosin (H&amp;E) staining and p16 immunostaining without knowledge of original diagnosis. Our primary outcomes were diagnostic agreement and test performance of p16 immunostaining compared to consensus H&amp;E diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our sample was comprised  of 23 cases of atypical genital skin lesions. p16 immunostaining was negative in all cases of reactive atypia (n = 3) and the majority (n = 7 of 8; 88%) of low\u2010grade squamous intraepithelial lesions (<jats:styled-content style=\"fixed-case\">LSILs<\/jats:styled-content>). The majority (n = 10 of 12; 83%) of high\u2010grade squamous intraepithelial lesions (<jats:styled-content style=\"fixed-case\">HSIL<\/jats:styled-content>) were p16 positive. Diagnostic agreement for histopathologic assessment using H&amp;E staining was moderate (kappa = 0.44), while inter\u2010observer agreement of p16 immunostaining was excellent (kappa = 0.87). Compared to consensus diagnosis using H&amp;E staining, p16 immunostaining performed well (sensitivity 83.3%; specificity 90.9%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>p16 immunostaining may be a useful adjunctive marker for assessing dysplasia in genital skin lesions and increasing diagnostic agreement among pathologists.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.12525","type":"journal-article","created":{"date-parts":[[2015,5,7]],"date-time":"2015-05-07T12:43:07Z","timestamp":1431002587000},"page":"519-526","source":"Crossref","is-referenced-by-count":5,"title":["Grading of atypia in genital skin lesions: routine microscopic evaluation and use of p16 immunostaining"],"prefix":"10.1111","volume":"42","author":[{"given":"Harib","family":"Ezaldein","sequence":"first","affiliation":[{"name":"Department of Dermatology Yale School of Medicine  New Haven CT USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason 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