{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T22:24:46Z","timestamp":1792189486921,"version":"4.4.0"},"reference-count":58,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T00:00:00Z","timestamp":1439164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Chronic cutaneous borreliosis (acrodermatitis chronica atrophicans, <jats:styled-content style=\"fixed-case\">ACA<\/jats:styled-content>) is a relatively rare manifestation of borreliosis attributed mainly to <jats:italic>Borrelia afzelii<\/jats:italic>. Chronic borreliosis has been associated with <jats:italic>ospA<\/jats:italic> and <jats:italic>ospC<\/jats:italic> genotypes. Literature on molecular investigations of <jats:italic>Borrelia<\/jats:italic> in lesions of <jats:styled-content style=\"fixed-case\">ACA<\/jats:styled-content> is scant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Histopathological and immmunohistochemical features in 22 biopsies of <jats:styled-content style=\"fixed-case\">ACA<\/jats:styled-content> (16 patients) were examined. Paraffin\u2010embedded biopsies were analyzed with polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) assays targeting <jats:italic>ospA<\/jats:italic> and <jats:italic>ospC<\/jats:italic> genes, sequencing and phylogenetic analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Genotyping of <jats:italic>ospA<\/jats:italic> identified <jats:italic>B. afzelii,<\/jats:italic> serotype 2, in 12 of 16 patients. <jats:italic>ospC<\/jats:italic>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> was positive in seven patients revealing genotypes Af5 (n\u2009=\u20094), Af2 (n\u2009=\u20092) and Af6 (n\u2009=\u20091). Histopathologically, interstitial granulomatous infiltrates (<jats:styled-content style=\"fixed-case\">CD68<\/jats:styled-content> positive) were common, combined with thickened collagen bundles and band\u2010like infiltrates of <jats:styled-content style=\"fixed-case\">CD4<\/jats:styled-content> positive T lymphocytes. Plasma cells were sparse\/absent in 9 of 22 specimens even on staining with <jats:styled-content style=\"fixed-case\">CD138<\/jats:styled-content>. On <jats:styled-content style=\"fixed-case\">CD34<\/jats:styled-content>\u2010staining, interstitial fibroblasts were often reduced akin to the situation in morphea.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>With assays targeting <jats:italic>ospA<\/jats:italic> and <jats:italic>ospC<\/jats:italic> genes we confirmed from paraffin\u2010embedded biopsies that <jats:italic>B. afzelii<\/jats:italic>, serotype 2, <jats:italic>osp C<\/jats:italic> groups Af5, Af2 and Af6 is the main cause of <jats:styled-content style=\"fixed-case\">ACA<\/jats:styled-content>. Specimens commonly showed a combination of band\u2010like T\u2010cell\u2010rich infiltrates with interstitial granulomatous features, a pattern previously under\u2010recognized in <jats:styled-content style=\"fixed-case\">ACA<\/jats:styled-content>. This finding was particularly typical for lesions infected with <jats:styled-content style=\"fixed-case\">ospC<\/jats:styled-content> genotype Af5.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.12550","type":"journal-article","created":{"date-parts":[[2015,7,8]],"date-time":"2015-07-08T06:48:34Z","timestamp":1436338114000},"page":"674-692","source":"Crossref","is-referenced-by-count":14,"title":["Histopathology and immunophenotype of acrodermatitis chronica atrophicans correlated with <i>ospA<\/i> and <i>ospC<\/i> genotypes of <i>Borrelia<\/i> species"],"prefix":"10.1111","volume":"42","author":[{"given":"Friederike C.","family":"Brandt","sequence":"first","affiliation":[{"name":"Department of Dermatology Munster University  M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beyhan","family":"Ertas","sequence":"additional","affiliation":[{"name":"Dermatologikum Hamburg  Hamburg 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