{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T16:42:13Z","timestamp":1750264933130},"reference-count":59,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,10,19]],"date-time":"2015-10-19T00:00:00Z","timestamp":1445212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004564","name":"Ministarstvo Prosvete, Nauke i Tehnolo\u0161kog Razvoja","doi-asserted-by":"publisher","award":["175026"],"award-info":[{"award-number":["175026"]}],"id":[{"id":"10.13039\/501100004564","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2016,3]]},"abstract":"Background<\/jats:title>Actinic keratosis (AK<\/jats:styled-content>) and Bowen's disease (squamous cell carcinoma in situ<\/jats:italic>, SCCIS<\/jats:styled-content>) are pre\u2010invasive stages in the development of squamous cell carcinoma (SCC<\/jats:styled-content>).<\/jats:p><\/jats:sec>Methods<\/jats:title>Immunohistochemical study of cyclin D1<\/jats:styled-content>, cyclin E, p16INK4a<\/jats:sup><\/jats:styled-content> and p21Cip1<\/jats:sup><\/jats:styled-content>\/Waf1<\/jats:sup> in AK<\/jats:styled-content> (53 cases), SCCIS<\/jats:styled-content> (16 cases) and SCC<\/jats:styled-content> (40 cases), in relation to the type of the lesion and SCC<\/jats:styled-content> prognostic parameters (grade, diameter and thickness).<\/jats:p><\/jats:sec>Results<\/jats:title>Diffuse cyclin D1<\/jats:styled-content> distribution was more frequent in SCCIS<\/jats:styled-content> and SCC<\/jats:styled-content> than in AK<\/jats:styled-content> (p\u2009=\u20090.03) and similar pattern was observed for p16INK4a<\/jats:sup><\/jats:styled-content>. For cyclin E, central distribution dominated in SCC<\/jats:styled-content> compared with the AK<\/jats:styled-content> (p\u2009=\u20090.001) and SCCIS<\/jats:styled-content> (p\u2009=\u20090.03). p21Cip1<\/jats:sup><\/jats:styled-content>\/Waf1<\/jats:sup> displayed suprabasal distribution more frequently in AK<\/jats:styled-content> than in SCCIS<\/jats:styled-content> (p\u2009=\u20090.001) and SCC<\/jats:styled-content> (p\u2009=\u20090.0004). Semiquantitative assessment showed more positive cells in AK<\/jats:styled-content> (p\u2009=\u20090.04) and SCCIS<\/jats:styled-content> (p\u2009=\u20090.04) than in SCC<\/jats:styled-content> for cyclin E. SCC<\/jats:styled-content> with diameter over 20\u2009mm and those thicker than 6\u2009mm revealed higher labeling index with p16INK4a<\/jats:sup><\/jats:styled-content> and p21Cip1<\/jats:sup><\/jats:styled-content>\/Waf1<\/jats:sup>, respectively.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Our results suggest different alterations for p16INK4a<\/jats:sup><\/jats:styled-content> and p21Cip1<\/jats:sup><\/jats:styled-content>\/Waf1<\/jats:sup> in AK<\/jats:styled-content>, SCCIS<\/jats:styled-content> and SCC<\/jats:styled-content>. Immunostaining distribution showed closer correlation with the type of the lesion, whereas percentage of positive cells displayed better association with the SCC<\/jats:styled-content> prognostic parameters.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.12623","type":"journal-article","created":{"date-parts":[[2015,9,9]],"date-time":"2015-09-09T09:04:46Z","timestamp":1441789486000},"page":"200-210","source":"Crossref","is-referenced-by-count":7,"title":["Expression of G1<\/scp>\/S\u2010cyclins and cyclin\u2010dependent kinase inhibitors in actinic keratosis and squamous cell carcinoma"],"prefix":"10.1111","volume":"43","author":[{"given":"Dimitrije","family":"Brasanac","sequence":"first","affiliation":[{"name":"Institute of Pathology Faculty of Medicine, University of Belgrade Belgrade Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jelena","family":"Stojkovic\u2010Filipovic","sequence":"additional","affiliation":[{"name":"Clinic of Dermatovenereology, Clinical 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