{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:20:01Z","timestamp":1770823201217,"version":"3.50.1"},"reference-count":14,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,7,28]],"date-time":"2016-07-28T00:00:00Z","timestamp":1469664000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Although syphilis is uncommon, infection rates are much higher in HIV\u2010infected individuals than the general population. A proposed explanation is impaired cellular immunity with HIV infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A search of one institution yielded 10 patients with a diagnosis of secondary syphilis on skin biopsy, positive syphilis serology and available CD4 counts. We evaluated 11 biopsies from the 10 patients. We correlated the patients' CD4 counts with the histologic findings and with the number of treponemes on skin biopsies, highlighted by immunohistochemistry (IHC). We also compared the detection of spirochetes in silver stained sections (e.g. Warthin\u2013Starry) with <jats:italic>T. pallidum<\/jats:italic> IHC. All biopsies were assessed for various histologic features.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The sensitivity of IHC to detect treponemes was 64% and of silver stain was 9% (p\u2010value 0.04). The number of treponemes on the biopsies was determined by IHC. High numbers of spirochetes (i.e. &gt;100 per 10 hpf) were only seen in patients with CD4 counts less than 250 cells\/ml.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The most consistent histologic finding was a moderate to severe lymphoplasmacytic infiltrate. Although the study is small, it appears that a higher number of spirochetes is associated with CD4 counts less than 250 cell\/ml. The <jats:italic>T. pallidum<\/jats:italic> IHC stain was vastly superior to the Warthin\u2013Starry stain.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.12756","type":"journal-article","created":{"date-parts":[[2016,6,15]],"date-time":"2016-06-15T13:28:20Z","timestamp":1465997300000},"page":"847-851","source":"Crossref","is-referenced-by-count":10,"title":["Secondary syphilis in HIV positive individuals: correlation with histopathologic findings, CD4 counts, and quantity of treponemes in microscopic sections"],"prefix":"10.1111","volume":"43","author":[{"given":"Gabriela","family":"Rosa","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Institute The Cleveland Clinic  Cleveland OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary W.","family":"Procop","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Institute The Cleveland Clinic  Cleveland OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jesse D.","family":"Schold","sequence":"additional","affiliation":[{"name":"Department of Quantitative Health Sciences The Cleveland Clinic  Cleveland OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melissa P.","family":"Piliang","sequence":"additional","affiliation":[{"name":"Sections of the Departments of Dermatology and Anatomic Pathology The Cleveland Clinic  Cleveland OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,7,28]]},"reference":[{"key":"e_1_2_5_2_1","unstructured":"CDC. 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