{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T01:26:35Z","timestamp":1776821195020,"version":"3.51.2"},"reference-count":39,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,9,15]],"date-time":"2016-09-15T00:00:00Z","timestamp":1473897600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Deane Endowment Trust"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2016,12]]},"abstract":"Background<\/jats:title>Recent description of hemangioblastic blood islands within pyogenic granuloma (PG<\/jats:styled-content>) has led us to investigate the expression of embryonic stem cell (ESC) markers in this tumor.<\/jats:p><\/jats:sec>Methods<\/jats:title>In this study we examined the expression of ESC<\/jats:styled-content> markers, OCT4<\/jats:styled-content>, SOX2<\/jats:styled-content>, STAT3<\/jats:styled-content> and NANOG<\/jats:styled-content> in PG<\/jats:styled-content> samples from 11 patients, by immunohistochemical (IHC<\/jats:styled-content>) staining, NanoString<\/jats:styled-content> analysis and in situ<\/jats:italic> hybridization (ISH<\/jats:styled-content>).<\/jats:p><\/jats:sec>Results<\/jats:title>IHC<\/jats:styled-content> staining demonstrated the expression of pSTAT3<\/jats:styled-content>, OCT4<\/jats:styled-content>, SOX2<\/jats:styled-content> and NANOG<\/jats:styled-content> by the endothelium of the microvessels in PG<\/jats:styled-content> whilst pSTAT3<\/jats:styled-content>, SOX2<\/jats:styled-content> and NANOG<\/jats:styled-content> were also expressed by cells in the interstitium, outside of the microvessels. NanoString<\/jats:styled-content> and ISH<\/jats:styled-content> analysis showed mRNA<\/jats:styled-content> expression for STAT3<\/jats:styled-content>, OCT4<\/jats:styled-content> and NANOG<\/jats:styled-content> in PG<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The expression of the ESC<\/jats:styled-content> markers, OCT4<\/jats:styled-content>, SOX2<\/jats:styled-content>, pSTAT3<\/jats:styled-content> and NANOG<\/jats:styled-content>, suggests the endothelium of PG<\/jats:styled-content> displays a primitive phenotype. Cells in the interstitium expressing pSTAT3<\/jats:styled-content>, SOX2<\/jats:styled-content> and NANOG<\/jats:styled-content> may represent a more downstream derivative of the primitive endothelium, or a separate population. The primitive nature of the endothelium and cells in the interstitium reveals novel insights into the biology of PG<\/jats:styled-content>. To the best of our knowledge, this is the first demonstration of the expression of ESC<\/jats:styled-content> markers in PG<\/jats:styled-content>, implying the presence of a hematopoietic stem cell population.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.12786","type":"journal-article","created":{"date-parts":[[2016,8,10]],"date-time":"2016-08-10T13:00:03Z","timestamp":1470834003000},"page":"1096-1101","source":"Crossref","is-referenced-by-count":16,"title":["Expression of embryonic stem cell markers in pyogenic granuloma"],"prefix":"10.1111","volume":"43","author":[{"given":"Max G.","family":"Blackwell","sequence":"first","affiliation":[{"name":"Gillies McIndoe Research Institute Wellington New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tinte","family":"Itinteang","sequence":"additional","affiliation":[{"name":"Gillies McIndoe Research Institute Wellington New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice M.","family":"Chibnall","sequence":"additional","affiliation":[{"name":"Gillies McIndoe Research Institute Wellington New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul F.","family":"Davis","sequence":"additional","affiliation":[{"name":"Gillies McIndoe Research Institute Wellington New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Swee T.","family":"Tan","sequence":"additional","affiliation":[{"name":"Gillies McIndoe Research Institute Wellington New Zealand"},{"name":"Centre for the Study & Treatment of Vascular Birthmarks, Wellington Regional Plastic, Maxillofacial & Burns Unit Hutt Hospital Wellington New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,9,15]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2014-3673"},{"key":"e_1_2_5_3_1","series-title":"A Volume in Foundations in Diagnostic Pathology Series","volume-title":"Head and Neck Pathology","author":"Thompson LD","year":"2012"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0030-4220(51)90432-X"},{"key":"e_1_2_5_5_1","volume-title":"Lever's Histopathology of the Skin","author":"Lever WF","year":"2009"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-198010000-00007"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-1470.1991.tb00931.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cps.2010.08.007"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/pin.12096"},{"key":"e_1_2_5_10_1","first-page":"467","article-title":"Clinical diagnosis and management of hormonally responsive oral pregnancy tumor (pyogenic granuloma)","volume":"41","author":"Sills E","year":"1996","journal-title":"J Reprod Med"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1067\/mjd.2000.104520"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joms.2009.07.070"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001665-200503000-00022"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjps.2010.12.021"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjps.2009.06.031"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5391.1145"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1050-1738(03)00125-7"},{"key":"e_1_2_5_18_1","unstructured":"Health NIo. NIH Human Embryonic Stem Cell Registry. 13 July 2011. URLhttp:\/\/grants.nih.gov\/stem_cells\/registry\/current.htm."},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81769-9"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M502573200"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08113"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(02)02352-8"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02787"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.224503"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.08.020"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2005.03.003"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M601811200"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.221176.113"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1151526"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000372-200010000-00009"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0303-6987.2004.00233.x"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2012.148"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2011-200462"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2014-202846"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e318206b67b"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.14.6031-6046.2005"},{"key":"e_1_2_5_37_1","first-page":"74","article-title":"The Oct4 protein: more than a magic stemness marker","volume":"3","author":"Zeineddine D","year":"2014","journal-title":"Am J Stem Cells"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1001-971"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.22508"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2010.079368"}],"container-title":["Journal of Cutaneous Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcup.12786","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cup.12786","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T19:33:47Z","timestamp":1695756827000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cup.12786"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,15]]},"references-count":39,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["10.1111\/cup.12786"],"URL":"https:\/\/doi.org\/10.1111\/cup.12786","archive":["Portico"],"relation":{},"ISSN":["0303-6987","1600-0560"],"issn-type":[{"value":"0303-6987","type":"print"},{"value":"1600-0560","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,15]]}}}