{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T13:25:22Z","timestamp":1744982722105},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,10,25]],"date-time":"2016-10-25T00:00:00Z","timestamp":1477353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2017,1]]},"abstract":"Background<\/jats:title>For selecting therapy, it is important to distinguish different types of keratinocytic neoplasia. It is sometimes difficult to make histopathologic diagnosis, especially in organ transplant recipients (OTR<\/jats:styled-content>) who develop numerous lesions.<\/jats:p><\/jats:sec>Methods<\/jats:title>To investigate p16 immunostaining in different types of keratinocytic neoplasia in OTR<\/jats:styled-content>, we studied 59 actinic keratoses (AK<\/jats:styled-content>), 51 Bowen' s disease (BD<\/jats:styled-content>), 63 squamous cell carcinomas (SCC<\/jats:styled-content>), 16 benign keratotic lesions (BKL<\/jats:styled-content>) from 31 OTR<\/jats:styled-content> patients and 25 controls (eczema and psoriasis). Tissue sections were stained for H&E and p16. We scored intensity, proportion and distribution of p16 positive lesional cells.<\/jats:p><\/jats:sec>Results<\/jats:title>In 19% of AK<\/jats:styled-content>, 92% of BD<\/jats:styled-content>, 35% of SCC<\/jats:styled-content> and 12% of BKL<\/jats:styled-content> more than 15% of lesional cells were p16\u2010positive. In 16% of AK<\/jats:styled-content>, 80% of BD<\/jats:styled-content>, 18% of SCC<\/jats:styled-content> and 13% of BKL<\/jats:styled-content> strong p16 staining was observed. BKL<\/jats:styled-content>, AK<\/jats:styled-content> and SCC<\/jats:styled-content> showed focal and patchy staining, BD<\/jats:styled-content> showed diffuse pattern with strong staining of all atypical cells. Sparing of the basal layer was predominantly seen in BD<\/jats:styled-content>. No control specimen showed p16\u2010overexpression.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>p16<\/jats:styled-content> immunostaining shows a characteristic pattern in BD<\/jats:styled-content>, but not in AK<\/jats:styled-content>, SCC<\/jats:styled-content> and BKL<\/jats:styled-content>. It appears useful in recognizing BD<\/jats:styled-content>, but not in differentiating between other keratinocytic neoplasia.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.12840","type":"journal-article","created":{"date-parts":[[2016,10,13]],"date-time":"2016-10-13T05:41:01Z","timestamp":1476337261000},"page":"28-33","source":"Crossref","is-referenced-by-count":4,"title":["p16 immunostaining in keratinocytic neoplasia in organ transplant recipients: Bowen's disease shows a characteristic pattern"],"prefix":"10.1111","volume":"44","author":[{"given":"Roel E.","family":"Genders","sequence":"first","affiliation":[{"name":"Department of Dermatology Leiden University Medical Center Leiden the Netherlands"},{"name":"Department of Dermatology Roosevelt Clinics Leiden The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel","family":"Beck","sequence":"additional","affiliation":[{"name":"DDL Diagnostic Laboratory Rijswijk the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan Nico","family":"Bouwes Bavinck","sequence":"additional","affiliation":[{"name":"Department of Dermatology Leiden University Medical Center Leiden the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henk A.M.","family":"van den Munckhof","sequence":"additional","affiliation":[{"name":"DDL Diagnostic Laboratory Rijswijk the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stijn T.P.","family":"Kouwenhoven","sequence":"additional","affiliation":[{"name":"Department of Dermatology Leiden University Medical Center Leiden the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maurits N.C.","family":"de Koning","sequence":"additional","affiliation":[{"name":"DDL Diagnostic Laboratory Rijswijk the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank R.","family":"de 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