{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T17:21:54Z","timestamp":1790443314267,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,1,26]],"date-time":"2017-01-26T00:00:00Z","timestamp":1485388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Divisions of Dermatology and Dermatopathology, Department of Pathology, Albany Medical College"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The expression of <jats:styled-content style=\"fixed-case\">TRPM1<\/jats:styled-content> (melastatin) <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> is an independent marker, as measured by radioactive in situ hybridization (<jats:styled-content style=\"fixed-case\">RISH<\/jats:styled-content>), of disease\u2010free survival in primary cutaneous melanoma (<jats:styled-content style=\"fixed-case\">PM<\/jats:styled-content>). The aim of the study was to determine if chromogenic in situ hybridization (<jats:styled-content style=\"fixed-case\">CISH<\/jats:styled-content>) can reproduce results examining diagnostic and prognostic utility of <jats:styled-content style=\"fixed-case\">TRPM1 mRNA<\/jats:styled-content> expression in melanocytic proliferations as measured by <jats:styled-content style=\"fixed-case\">RISH<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>\n<jats:styled-content style=\"fixed-case\">Methods<\/jats:styled-content>\n<\/jats:title><jats:p>The expression of <jats:styled-content style=\"fixed-case\">TRPM1 mRNA<\/jats:styled-content> was detected by <jats:styled-content style=\"fixed-case\">CISH<\/jats:styled-content> in melanocytic nevi (<jats:styled-content style=\"fixed-case\">MN<\/jats:styled-content>, <jats:italic>n<\/jats:italic>\u2009=\u200961), <jats:styled-content style=\"fixed-case\">PM<\/jats:styled-content> (<jats:italic>n<\/jats:italic>\u2009=\u2009145) and metastatic melanomas (<jats:styled-content style=\"fixed-case\">MMs<\/jats:styled-content>, <jats:italic>n<\/jats:italic>\u2009=\u200915).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A progressive loss of <jats:styled-content style=\"fixed-case\">TRPM1<\/jats:styled-content> was found moving from <jats:styled-content style=\"fixed-case\">MN<\/jats:styled-content> to <jats:styled-content style=\"fixed-case\">PM<\/jats:styled-content> to <jats:styled-content style=\"fixed-case\">MM<\/jats:styled-content>. The histologic stepwise model of melanoma progression revealed that loss of <jats:styled-content style=\"fixed-case\">TRPM1<\/jats:styled-content> occurred at the transition of <jats:styled-content style=\"fixed-case\">RGP PM<\/jats:styled-content> to <jats:styled-content style=\"fixed-case\">VGP PM<\/jats:styled-content>. As a diagnostic marker, <jats:styled-content style=\"fixed-case\">TRPM1<\/jats:styled-content> gradient loss showed 93.8% sensitivity and 52.4% specificity for <jats:styled-content style=\"fixed-case\">PM<\/jats:styled-content>. Loss of <jats:styled-content style=\"fixed-case\">TRPM1 mRNA<\/jats:styled-content> correlated with melanoma aggressiveness markers and was independent predictor of disease\u2010free and overall survival. The corresponding survival curves for degree of melanoma pigmentation matched those for degree of loss of <jats:styled-content style=\"fixed-case\">TPRM1 mRNA<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Loss of <jats:styled-content style=\"fixed-case\">TRPM1 mRNA<\/jats:styled-content> expression appears to be a crucial event in the progression of melanoma to a more malignant, metastatic phenotype.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.12872","type":"journal-article","created":{"date-parts":[[2016,12,17]],"date-time":"2016-12-17T17:34:41Z","timestamp":1481996081000},"page":"328-337","source":"Crossref","is-referenced-by-count":17,"title":["<scp>TRPM1<\/scp> (melastatin) expression is an independent predictor of overall survival in clinical <scp>AJCC<\/scp> stage I and <scp>II<\/scp> melanoma patients"],"prefix":"10.1111","volume":"44","author":[{"given":"Anna A.","family":"Bro\u017cyna","sequence":"first","affiliation":[{"name":"Department of Tumor Pathology and Pathomorphology, Oncology Centre\u2014Prof. Franciszek \u0141ukaszczyk Memorial Hospital, Department of Tumor Pathology and Pathomorphology, Faculty of Health Sciences Nicolaus Copernicus University Collegium Medicum in Bydgoszcz  Bydgoszcz Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huazhang","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Pittsburgh Medical Center, UPMC Cancer Pavilion  Pittsburgh Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun\u2010Eun","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathology Albany Medical College MC\u201081  Albany New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynn","family":"Cornelius","sequence":"additional","affiliation":[{"name":"Division of Dermatology, Washington University School of Medicine  St. Louis Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald","family":"Linette","sequence":"additional","affiliation":[{"name":"Division of Oncology, Washington University School of Medicine  St. Louis Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Murphy","sequence":"additional","affiliation":[{"name":"Department of Dermatology, MC\u20106230 University of Connecticut Health Center  Farmington Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Sheehan","sequence":"additional","affiliation":[{"name":"Department of Pathology Albany Medical College MC\u201081  Albany New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Ross","sequence":"additional","affiliation":[{"name":"Department of Pathology Albany Medical College MC\u201081  Albany New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Slominski","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Alabama at Birmingham  Birmingham Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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