{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T07:14:10Z","timestamp":1767597250731,"version":"3.37.3"},"reference-count":46,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,4,10]],"date-time":"2017-04-10T00:00:00Z","timestamp":1491782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Chronic amastigote\u2010negative cutaneous leishmaniasis (<jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content>) is a diagnostic challenge, as the parasite load may be low, or absent in biopsy tissue sections.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A series of consecutive biopsy specimens, taken from 130 patients with a diagnosis of granulomatous dermatitis of unknown etiology, were reviewed. Polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) was carried out for Leishmania\u2010specific <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 27 of 130 samples were positive for Leishmania\u2010specific <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>. In only 3 patients was a clinical diagnosis <jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content> made. The lesions were, single or multiple nodules or plaques of many months duration. Histopathologically, a tuberculoid granulomatous dermatitis was the least common denominator in every case, whilst in 5 cases a heavy lymphoid component was predominant. One patient had a concurrent cutaneous marginal zone lymphoma (<jats:styled-content style=\"fixed-case\">MZL<\/jats:styled-content>), the additional <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> study showed the presence of Leishmania <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> in tissue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results of this study expand on previous observations as to the deceptive clinicopathologic manifestations of chronic <jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content>, confirming the diagnostic value of <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> analysis for Leishmania <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> in unspecified granulomatous dermatitides. We also suggest that, in countries where Leishmaniasis is endemic, <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> for Leishmania\u2010specific <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> be performed in any idiopathic pseudolymphomatous. More compelling evidence as to whether chronic Leishmania infection is implicated in the pathogenesis of some cutaneous <jats:styled-content style=\"fixed-case\">MZL<\/jats:styled-content> is warranted by further studies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.12927","type":"journal-article","created":{"date-parts":[[2017,3,10]],"date-time":"2017-03-10T17:08:47Z","timestamp":1489165727000},"page":"530-537","source":"Crossref","is-referenced-by-count":12,"title":["Chronic amastigote\u2010negative cutaneous leishmaniasis: A clinical, histopathologic and molecular study of 27 cases with emphasis on atypical and pseudolymphomatous presentations"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8383-3443","authenticated-orcid":false,"given":"Carlo","family":"Tomasini","sequence":"first","affiliation":[{"name":"Dermatopathology Unit Azienda Ospedaliero\u2010Universitaria Citt\u00e0 della Salute e della Scienza  Turin Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Moneghini","sequence":"additional","affiliation":[{"name":"Division of Pathology, Department of Health Sciences University of Milan, Hospital San Paolo  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna M.","family":"Barbui","sequence":"additional","affiliation":[{"name":"Microbiology and Virology Unit Azienda Ospedaliero Universitaria Citt\u00e0 della Salute e della Scienza  Turin Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,4,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67629-5"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2012.03.005"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035671"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(07)70209-8"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES2013.18.29.20535"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0129418"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES2013.18.30.20546"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2010.06.019"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES2013.18.30.20544"},{"key":"e_1_2_6_11_1","doi-asserted-by":"crossref","first-page":"20205","DOI":"10.2807\/ese.17.26.20205-en","article-title":"Infectious diseases among travellers and migrants in Europe, EuroTravNet 2010","volume":"17","author":"Gautret P","year":"2012","journal-title":"Euro Surveill"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365540903104127"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.2807\/ese.15.10.19505-en"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1407.071589"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0738-081X(99)00047-4"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(87)70259-X"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4362.1988.tb01268.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.13812"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2000.03909.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0738-081X(99)00048-6"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711410109"},{"key":"e_1_2_6_22_1","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1001\/archderm.1996.03890260100015","article-title":"Chronic lupoid leishmaniasis","volume":"132","author":"Momeni AZ","year":"1996","journal-title":"Arch Dermatol"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.2011.01844.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2006.07365.x"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1159\/000063150"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(03)00093-2"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0365-05962011000500005"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2013-0098-OA"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.14088"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2009.09282.x"},{"issue":"3","key":"e_1_2_6_31_1","first-page":"256","article-title":"Chronic lupoid leishmaniasis. 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