{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T06:17:10Z","timestamp":1792304230106,"version":"4.4.0"},"reference-count":18,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,6,19]],"date-time":"2017-06-19T00:00:00Z","timestamp":1497830400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2017,9]]},"abstract":"Background<\/jats:title>Sentinel lymph node (SLN<\/jats:styled-content>) metastasis is a powerful predictor of survival in primary cutaneous melanoma. Lymphatic invasion (LI<\/jats:styled-content>) may correlate with increased risk of SLN<\/jats:styled-content> metastasis. Intralymphatic metastases, often difficult to detect on hematoxylin and eosin (H&E) stained sections, are readily identified with dual immunohistochemistry for melanocytic and lymphatic markers.<\/jats:p><\/jats:sec>Methods<\/jats:title>We used dual S100<\/jats:styled-content>\/D240<\/jats:styled-content> immunohistochemistry to detect LI<\/jats:styled-content> in 125 melanomas from patients who underwent SLN<\/jats:styled-content> biopsy and correlated LI<\/jats:styled-content> with melanoma staging parameters and disease status.<\/jats:p><\/jats:sec>Results<\/jats:title>Dual immunohistochemistry allowed for the identification of LI<\/jats:styled-content> in 33 cases (26%), compared to only 2% on H&E stained sections. Melanomas with LI<\/jats:styled-content> showed greater thickness, higher mitotic rate and more frequent ulceration. Eleven of 33 cases with LI<\/jats:styled-content> (33%) and 10 of 92 cases without LI<\/jats:styled-content> (11%) were associated with a positive SLN<\/jats:styled-content> (P<\/jats:italic>\u2009=\u2009.006). More patients without LI<\/jats:styled-content> were disease\u2010free at last follow\u2010up (80%) than patients with LI<\/jats:styled-content> (50%; P<\/jats:italic>\u2009=\u2009.002); LI<\/jats:styled-content> was significantly associated with decreased progression\u2010free survival.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>The detection of LI<\/jats:styled-content> is improved by dual immunohistochemistry and predicts SLN<\/jats:styled-content> metastasis. The presence of LI<\/jats:styled-content> may impact therapeutic planning in melanoma, such as the decision to perform a SLN<\/jats:styled-content> biopsy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.12969","type":"journal-article","created":{"date-parts":[[2017,5,29]],"date-time":"2017-05-29T08:26:44Z","timestamp":1496046404000},"page":"734-739","source":"Crossref","is-referenced-by-count":16,"title":["Lymphatic invasion predicts sentinel lymph node metastasis and adverse outcome in primary cutaneous melanoma"],"prefix":"10.1111","volume":"44","author":[{"given":"Andrea P","family":"Moy","sequence":"first","affiliation":[{"name":"Pathology Service Massachusetts General Hospital Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark C","family":"Mochel","sequence":"additional","affiliation":[{"name":"Pathology Service Massachusetts General Hospital Boston Massachusetts"},{"name":"Department of Pathology Virginia Commonwealth University Richmond Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alona","family":"Muzikansky","sequence":"additional","affiliation":[{"name":"Biostatistics Center Massachusetts General Hospital and Harvard Medical School Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lyn M","family":"Duncan","sequence":"additional","affiliation":[{"name":"Pathology Service Massachusetts General Hospital Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0912-7895","authenticated-orcid":false,"given":"Stefan","family":"Kraft","sequence":"additional","affiliation":[{"name":"Pathology Service Massachusetts General Hospital Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,6,19]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2001.19.16.3622"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMe068147"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.144.4.462"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.2008.01242.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1177\/000313481107700816","article-title":"Lymphovascular invasion as a prognostic factor in melanoma","volume":"77","author":"Egger ME","year":"2011","journal-title":"Am Surg"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2010.03.005"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-11-0490"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2011.182"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2009.04.026"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31822573f5"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2007.10.017"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.2008.01166.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2014.11.006"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2016.131"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0000000000000453"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e3182036ccd"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e318288ff47"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12688"}],"container-title":["Journal of Cutaneous Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcup.12969","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cup.12969","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T10:29:41Z","timestamp":1695551381000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cup.12969"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6,19]]},"references-count":18,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1111\/cup.12969"],"URL":"https:\/\/doi.org\/10.1111\/cup.12969","archive":["Portico"],"relation":{},"ISSN":["0303-6987","1600-0560"],"issn-type":[{"value":"0303-6987","type":"print"},{"value":"1600-0560","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6,19]]}}}