{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T16:59:16Z","timestamp":1782493156426,"version":"3.54.5"},"reference-count":13,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,9,15]],"date-time":"2017-09-15T00:00:00Z","timestamp":1505433600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2017,12]]},"abstract":"Background<\/jats:title>Positive CD1a<\/jats:styled-content> staining of Leishmania has been reported in Old World leishmaniasis, but the sensitivity of such staining for other Leishmania species is unknown.<\/jats:p><\/jats:sec>Methods<\/jats:title>A retrospective review was done on skin biopsies of proven cutaneous leishmaniasis based on histology, immunohistochemistry, culture and\/or polymerase chain reaction (PCR<\/jats:styled-content>). We assessed the pattern of inflammation present and assessed for CD1a<\/jats:styled-content> (MTB1<\/jats:styled-content> clone) positivity in amastigotes. Patients without a clearly documented travel history to delineate Old vs New World leishmaniasis and cases without tissue for CD1a<\/jats:styled-content> staining were excluded.<\/jats:p><\/jats:sec>Results<\/jats:title>Various patterns of granulomatous inflammation were observed including sarcoidal (31%), diffuse (25%), suppurative and granulomatous (25%), palisaded (13%) and lichenoid (6%). CD1a<\/jats:styled-content> staining was positive in amastigotes in 9 of 16 cases (56%). Five of 7 (71%) cases of Old World disease were CD1a<\/jats:styled-content> positive, while 4 of 9 cases (44%) of New World cases were positive.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Multiple patterns of granulomatous inflammation occur in cutaneous leishmaniasis. Our results confirm CD1a<\/jats:styled-content> (MTB1<\/jats:styled-content> clone) can be a diagnostic adjunct to highlight amastigotes in biopsies of cutaneous leishmaniasis, with variable positivity in both Old World and New World forms of the disease. As 44% of cases were CD1a<\/jats:styled-content> negative in our cohort, there are significant limitations to this screening approach.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.13032","type":"journal-article","created":{"date-parts":[[2017,9,11]],"date-time":"2017-09-11T15:00:41Z","timestamp":1505142041000},"page":"1005-1011","source":"Crossref","is-referenced-by-count":34,"title":["Histopathologic features of cutaneous leishmaniasis and use of CD1a staining for amastigotes in Old World and New World leishmaniasis"],"prefix":"10.1111","volume":"44","author":[{"given":"Lohini","family":"Sundharkrishnan","sequence":"first","affiliation":[{"name":"Department of Pathology Orlando Health Orlando Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3973-5314","authenticated-orcid":false,"given":"Jeffrey P.","family":"North","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of California San Francisco San Francisco California"},{"name":"Department of Pathology University of California San Francisco San Francisco California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-4362.1997.00149.x"},{"key":"e_1_2_6_3_1","unstructured":"Parasites: Leishmaniasis. 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