{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T14:24:39Z","timestamp":1778509479408,"version":"3.51.4"},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,3,14]],"date-time":"2018-03-14T00:00:00Z","timestamp":1520985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Tumor infiltrating lymphocytes (TILs) represent important regulators of carcinogenesis. Cutaneous invasive squamous cell carcinoma (inSCC) develops through precursor lesions, namely in situ squamous cell carcinoma (isSCC) and actinic keratosis (AK), representing a natural model of carcinogenesis. The study evaluates TIL subpopulations in inSCC and its precursors by comparing 2 semiquantitative scoring systems, and assesses the presence of regulatory T\u2010cells (Tregs) in these lesions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Paraffin sections from 33 cases of AK, 19 isSCCs and 34 inSCCs with adjacent precursor lesions or normal skin (NS) were immunostained for CD3, CD4, CD8 and Foxp3. TIL subgroups were evaluated by the semiquantitative Klintrup\u2010M\u00e4kinen (K\u2010M) score, and by a more detailed modification of this system. Treg counts were assessed by image analysis quantification.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>An increase of all TIL subpolulations from precursor lesions toward inSCC was shown by both scoring systems. Treg counts progressively increased from NS to AK and isSCC, but decreased in inSCC. Tregs were more numerous in pT2 and around indolent inSCCs compared to T1 and aggressive subtypes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>T\u2010cells and cytotoxic T\u2010cells progressively increase in cutaneous squamous cell carcinogenesis, while Treg counts diminish in inSCC. The K\u2010M score is an appropriate, easily applicable TIL scoring system in cutaneous inSCC.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.13123","type":"journal-article","created":{"date-parts":[[2018,2,8]],"date-time":"2018-02-08T14:07:23Z","timestamp":1518098843000},"page":"337-347","source":"Crossref","is-referenced-by-count":17,"title":["Evaluation of T\u2010lymphocyte subpopulations in actinic keratosis, in situ and invasive squamous cell carcinoma of the skin"],"prefix":"10.1111","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8838-8334","authenticated-orcid":false,"given":"Aristea","family":"Stravodimou","sequence":"first","affiliation":[{"name":"Department of Pathology, School of Medicine University of Patras  Patras Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vassiliki","family":"Tzelepi","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine University of Patras  Patras Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen","family":"Papadaki","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Medicine University of Patras  Patras Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Athanasia","family":"Mouzaki","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Internal Medicine, School of Medicine University of Patras  Patras Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophia","family":"Georgiou","sequence":"additional","affiliation":[{"name":"Department of Dermatology, School of Medicine University of Patras  Patras Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Melachrinou","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine University of Patras  Patras Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eleni P.","family":"Kourea","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine University of Patras  Patras Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2005.12.005"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.01.025"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12307-010-0044-5"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19850715)56:2<413::AID-CNCR2820560234>3.0.CO;2-T"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19960401)77:7<1303::AID-CNCR12>3.0.CO;2-5"},{"key":"e_1_2_7_7_1","unstructured":"Smoller BR Balch C Crowson AN et al. 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