{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:41:40Z","timestamp":1772160100872,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,7,31]],"date-time":"2018-07-31T00:00:00Z","timestamp":1532995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Actinic keratosis (AK) and squamous cell carcinoma in\u2010situ (SCCIS) within or near melanoma in situ (MIS) can complicate diagnosis due to overlapping clinical and microscopic features. This study aimed to describe basilar melanocyte density and pagetoid spread in AK and SCCIS for improved diagnostic accuracy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 22 AK and 22 SCCIS biopsies containing a margin of uninvolved epidermis were immunostained with MART\u20101 (melanoma antigen recognized by T\u2010cells 1). The basilar melanocyte:keratinocyte ratio and the number and distribution of pagetoid melanocytes were compared in AK, SCCIS, and uninvolved epidermis. An in\u2010vitro human skin model was created to assess the impact of keratinocyte atypia on melanocyte distribution.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The median basilar melanocyte:keratinocyte ratio in SCCIS (1:11.49) was lower than in uninvolved epidermis (1:5.59,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.0011), and the ratio in AK (1:6.94) was similar to uninvolved epidermis (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.987). Pagetoid melanocytes were absent in perilesional skin but common in AK (21\/22,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.0001) and SCCIS (22\/22,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.0001). Pagetoid melanocytes at or above the mid\u2010spinous layer were more common in SCCIS (21\/22) vs AK (7\/22,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.0001). Pagetoid melanocytes were present in the in\u2010vitro skin model made with neoplastic but not normal keratinocytes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Pagetoid melanocytes in AK and SCCIS should be interpreted with caution to avoid overdiagnosis of MIS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cup.13309","type":"journal-article","created":{"date-parts":[[2018,6,26]],"date-time":"2018-06-26T00:20:32Z","timestamp":1529972432000},"page":"734-742","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["MART\u20101\u2010labeled melanocyte density and distribution in actinic keratosis and squamous cell cancer in situ: Pagetoid melanocytes are a potential source of misdiagnosis as melanoma in situ"],"prefix":"10.1111","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3984-4740","authenticated-orcid":false,"given":"William C.","family":"Fix","sequence":"first","affiliation":[{"name":"Department of Dermatology University of Pennsylvania, Perelman School of Medicine  Philadelphia Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4229-5831","authenticated-orcid":false,"given":"Sook Jung","family":"Yun","sequence":"additional","affiliation":[{"name":"Department of Dermatology Chonnam National University Medical School  Gwangju South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline M.","family":"Groft MacFarlane","sequence":"additional","affiliation":[{"name":"Bryn Mawr Medical Specialists Association  Bryn Mawr Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anokhi","family":"Jambusaria","sequence":"additional","affiliation":[{"name":"Division of Dermatology, Department of Internal Medicine University of Texas Austin, Dell Medical School  Austin Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosalie","family":"Elenitsas","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hospital of the University of Pennsylvania  Philadelphia Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5625-2782","authenticated-orcid":false,"given":"Emily","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hospital of the University of Pennsylvania  Philadelphia Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy R.","family":"Etzkorn","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hospital of the University of Pennsylvania  Philadelphia Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph F.","family":"Sobanko","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hospital of the University of Pennsylvania  Philadelphia Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thuzar M.","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hospital of the University of Pennsylvania  Philadelphia Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher J.","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hospital of the University of Pennsylvania  Philadelphia 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