{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:31:03Z","timestamp":1759332663153,"version":"3.40.5"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,12,17]],"date-time":"2018-12-17T00:00:00Z","timestamp":1545004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Techniques for the accurate identification of activating mutations of BRAF in metastatic melanoma are of great clinical importance, due to the availability of targeted therapies for these tumors. There is uncertainty regarding the frequency with which BRAF status differs between primary and metastatic sites.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Between 2011 and 2016, 219 melanoma cases underwent BRAF testing in our institution. In 53 of these cases, paired primary and metastatic specimens were available for polymerase chain reaction (PCR) and immunohistochemical evaluation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fifty\u2010two out of 53 cases (98%) showed concordant BRAF status between primary and metastatic site by immunohistochemistry (IHC). In one case, a metastasis and its matched primary were positive by IHC, but the metastasis was negative on PCR. On further investigation, PCR was positive in the primary, and repeat PCR in the metastasis was positive, following macrodissection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results suggest that discordance of BRAF mutational status between primaries and metastases is a rare occurrence. In one case, IHC provided strong evidence that initial PCR testing had provided a false\u2010negative result due to low tumor volume. Thus, in cases where tissue is difficult to obtain from a metastasis or unavailable, the primary tumor can be used with confidence.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.13393","type":"journal-article","created":{"date-parts":[[2018,11,15]],"date-time":"2018-11-15T08:41:12Z","timestamp":1542271272000},"page":"117-122","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["High concordance of BRAF mutational status in matched primary and metastatic melanoma"],"prefix":"10.1111","volume":"46","author":[{"given":"David","family":"Cormican","sequence":"first","affiliation":[{"name":"Department of Pathology Cork University Hospital  Cork Ireland"},{"name":"Department of Histopathology School of Medicine, Trinity College  Dublin Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ciaran","family":"Kennedy","sequence":"additional","affiliation":[{"name":"Department of Pathology Cork University Hospital  Cork Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Murphy","sequence":"additional","affiliation":[{"name":"Department of Pathology Cork University Hospital  Cork Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reiltin","family":"Werner","sequence":"additional","affiliation":[{"name":"Department of Pathology Cork University Hospital  Cork Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Derek G.","family":"Power","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Cork University Hospital  Cork Ireland"},{"name":"Department of Medicine School of Medicine, University College Cork  Cork Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2234-3739","authenticated-orcid":false,"given":"Cynthia C. 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