{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T04:51:56Z","timestamp":1780548716866,"version":"3.54.1"},"reference-count":10,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,5,3]],"date-time":"2019-05-03T00:00:00Z","timestamp":1556841600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Immunohistochemical (IHC) stains that distinguish benign, pigmented nail lesions from malignancy are needed. Candidate markers of malignant transformation include p16, HMB45, and Ki\u201067, with p16 being of particular interest. There is limited knowledge about the spectrum of p16 expression in pigmented lesions, especially junctional melanocytic proliferations of the nail. The objective of this study was to determine if any of these markers demonstrate diagnostic utility in distinguishing between benign activation of junctional melanocytes (BAM) and melanoma in situ (MIS) of the nail unit.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this retrospective study, ten cases of BAM and eight cases of MIS were identified. Archival slides available for review included H&amp;E (hematoxylin and eosin), Fontana\u2010Masson, and MelanA (Mart1) IHC slides. IHC studies for p16, HMB45, and dual\u2010color Ki\u201067\/MelanA (Mart1) were then performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>None of the tested IHC stains distinguished BAM from MIS. p16 IHC expression was uniformly negative with the exception of two cases of MIS. HMB45 was positive in all BAM and MIS cases. Ki\u201067\/MelanA showed positive Ki\u201067 staining of MelanA\u2010positive melanocytes in two cases of MIS, and all other cases of MIS and BAM were negative for Ki\u201067. The two positive p16 and two positive Ki\u201067\/MelanA cases were non\u2010overlapping.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>p16, HMB45, and Ki\u201067\/MelanA IHC studies show no apparent utility in distinguishing BAM from MIS in the nail unit.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.13457","type":"journal-article","created":{"date-parts":[[2019,3,20]],"date-time":"2019-03-20T00:28:57Z","timestamp":1553041737000},"page":"479-483","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Immunohistochemical characterization of benign activation of junctional melanocytes and melanoma in situ of the nail unit"],"prefix":"10.1111","volume":"46","author":[{"given":"Alex","family":"Chu","sequence":"first","affiliation":[{"name":"Department of Dermatology, Pathology and the Knight Cancer Institute Oregon Health &amp; Sciences University  Portland Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josette","family":"Andr\u00e9","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Hospitals Saint\u2010Pierre  Brussels Belgium"},{"name":"Department of Dermatopathology University Hospital Brugmann  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phoebe","family":"Rich","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Pathology and the Knight Cancer Institute Oregon Health &amp; Sciences University  Portland Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sancy","family":"Leachman","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Pathology and the Knight Cancer Institute Oregon Health &amp; Sciences University  Portland Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0788-346X","authenticated-orcid":false,"given":"Curtis T.","family":"Thompson","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Pathology and the Knight Cancer Institute Oregon Health &amp; Sciences University  Portland Oregon"},{"name":"Department of Dermatology, CTA Lab  Portland Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,3]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/archdermatol.2010.27"},{"key":"e_1_2_5_3_1","volume-title":"The Pigmentary System: Physiology and Pathophysiology","author":"King R","year":"2006"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31815c8578"},{"issue":"23","key":"e_1_2_5_5_1","first-page":"5475","article-title":"The p16\u2010cyclin D\/Cdk4\u2010pRb pathway as a functional unit frequently altered in melanoma pathogenesis","volume":"56","author":"Bartkova J","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/35092584"},{"issue":"10","key":"e_1_2_5_7_1","first-page":"1889","article-title":"Frequent alteration of CDKN2 (p16 (INK4A)\/MTS1) expression in human primary prostate carcinomas","volume":"3","author":"Chi SG","year":"1997","journal-title":"Clin Cancer Res"},{"issue":"20","key":"e_1_2_5_8_1","first-page":"4525","article-title":"Inactivation of the CDKN2\/p16\/MTS1 gene is frequently associated with aberrant DNA methylation in all common human cancers","volume":"55","author":"Herman JG","year":"1995","journal-title":"Cancer Res"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1095-210"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1827.2002.01359.x"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e3181e84652"}],"container-title":["Journal of Cutaneous Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cup.13457","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cup.13457","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cup.13457","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T17:56:31Z","timestamp":1694368591000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cup.13457"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,3]]},"references-count":10,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["10.1111\/cup.13457"],"URL":"https:\/\/doi.org\/10.1111\/cup.13457","archive":["Portico"],"relation":{},"ISSN":["0303-6987","1600-0560"],"issn-type":[{"value":"0303-6987","type":"print"},{"value":"1600-0560","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,3]]},"assertion":[{"value":"2018-09-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}