{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T15:25:01Z","timestamp":1785684301698,"version":"3.56.0"},"reference-count":33,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T00:00:00Z","timestamp":1590624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Determination of <jats:italic>BRAF<\/jats:italic> status is mandatory for targeted therapy but it is not currently available in most developing countries.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>We aimed to analyze <jats:italic>BRAF<\/jats:italic> mutations in a series of cutaneous melanoma of Tunisian patients and to compare <jats:italic>BRAF<\/jats:italic> V600E mutation detection by immunohistochemistry to DNA sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and methods<\/jats:title><jats:p>Archival formalin fixed paraffin embedded tissues from 28 patients with primary cutaneous melanoma were evaluated for the <jats:italic>BRAF<\/jats:italic> mutations by Sanger sequencing and immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>BRAF<\/jats:italic> mutations were detected in 19\/28 (68%) of analyzed tumors. The sensitivity and specificity of immunohistochemistry compared to DNA sequencing for identification of the <jats:italic>BRAF<\/jats:italic> V600E mutation were 100%. We found five novel mutations, one of them had deleterious effect.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The present study shows an intermediate frequency of mutations of the <jats:italic>BRAF<\/jats:italic> gene in cutaneous melanoma of Tunisian patients, and supports the use of immunohistochemistry as a screening test for the assessment of the <jats:italic>BRAF<\/jats:italic> V600E status in the management of melanoma in clinical practice.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.13710","type":"journal-article","created":{"date-parts":[[2020,4,14]],"date-time":"2020-04-14T01:30:10Z","timestamp":1586827810000},"page":"794-799","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Molecular and immunohistochemical analysis of <scp><i>BRAF<\/i><\/scp> gene in primary cutaneous melanoma: Discovery of novel 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