{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:47:45Z","timestamp":1787014065252,"version":"build-2736575974"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T00:00:00Z","timestamp":1634774400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Pityriasis lichenoides (PL) is a papulosquamous disease that affects both adults and children. Previous studies have shown a subset of this entity to have clonal T\u2010cell populations via PCR\u2010based assays. In this study, we sought to implement next\u2010generation sequencing (NGS) as a more sensitive and specific test to examine for T\u2010cell clonality within the pediatric population.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We identified 18 biopsy specimens from 12 pediatric patients with clinical and histopathologic findings compatible with PL. Patient demographics, clinical features, management, and histopathologic findings were reviewed. All specimens were analyzed for clonality with NGS of T\u2010cell receptor beta (\n                      <jats:italic>TRB<\/jats:italic>\n                      ) and gamma (\n                      <jats:italic>TRG<\/jats:italic>\n                      ) genes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Of the 12 patients, 9 (75%) had complete resolution of lesions at the time of data collection (mean follow\u2010up 31\u00a0months). The remaining three patients significantly improved with methotrexate (with or without acitretin). Interestingly, 7 of 12 patients (58%) and 9 of 17 biopsy specimens (53%) showed evidence of T\u2010cell clonality. Two patients showed matching\n                      <jats:italic>TRB<\/jats:italic>\n                      clones from different anatomic sites.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>T\u2010cell clonality is a common finding in PL, probably representing a \u201creactive clonality\u201d rather than a true lymphoproliferative disorder. Clonality alone cannot be used as a means to distinguish PL from lymphomatoid papulosis or cutaneous lymphoma.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cup.14143","type":"journal-article","created":{"date-parts":[[2021,10,8]],"date-time":"2021-10-08T10:23:23Z","timestamp":1633688603000},"page":"252-260","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Next\u2010generation sequencing confirms T\u2010cell clonality in a subset of pediatric pityriasis lichenoides"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3816-1542","authenticated-orcid":false,"given":"Shyam S.","family":"Raghavan","sequence":"first","affiliation":[{"name":"Department of Pathology University of Virginia Medical Center  Charlottesville Virginia 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