{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T18:32:57Z","timestamp":1789237977510,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T00:00:00Z","timestamp":1657584000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2022,10]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>Poromas, and their malignant counterparts, porocarcinomas, harbor recurrent translocations involving YAP1\u2010MAML2<\/jats:italic>, YAP1\u2010NUTM1<\/jats:italic>, and infrequently WWTR1\u2010NUTM1<\/jats:italic>; YAP1\u2010NUTM1<\/jats:italic> being the most common in porocarcinomas. NUT immunohistochemistry (IHC) can be used to identify NUTM1<\/jats:italic>\u2010translocated tumors. This study sought to investigate potential novel NUTM1<\/jats:italic>\u2010fusion partners among NUT IHC\u2010positive poromas and porocarcinomas.<\/jats:p><\/jats:sec>Methods<\/jats:title>Thirteen NUT IHC<\/jats:styled-content>\u2010positive poroid tumors (four poromas and nine porocarcinomas) were identified within a multi\u2010institutional international cohort. Next\u2010generation sequencing (NGS<\/jats:styled-content>) assessed for NUTM1<\/jats:italic><\/jats:styled-content> fusion partners.<\/jats:p><\/jats:sec>Results<\/jats:title>NGS detected a NUTM1<\/jats:italic> fusion in 12 of 13 cases: YAP1\u2010NUTM1<\/jats:italic> (11\/12 cases) and WWTR1\u2010NUTM1<\/jats:italic> (1\/12 cases). Two of the cases (2\/12) with NUTM1<\/jats:italic> fusion were not called by the NGS algorithm but had at least one read\u2010spanning YAP1\u2010NUTM1<\/jats:italic> break point upon manual review. A NUTM1<\/jats:italic> fusion was not identified in one case; however, the sample had low RNA quality. The following fusion events were identified: YAP1<\/jats:italic> exon 4::NUTM1<\/jats:italic> exon 3 in six cases, YAP1<\/jats:italic> exon 6::NUTM1<\/jats:italic> exon 2 in one case, YAP1<\/jats:italic> exon 3::NUTM1<\/jats:italic> exon 3 in three cases, WWTR1<\/jats:italic> exon 3::NUTM1<\/jats:italic> exon 3 in one case, and YAP1<\/jats:italic> exon 8::NUTM1<\/jats:italic> exon 3 fusion in one case.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>While no novel NUTM1<\/jats:italic> fusion partners were identified within our cohort, 12 of 13 cases had discoverable NUTM1<\/jats:italic> fusions; YAP1\u2010NUTM1<\/jats:italic> fusion was detected in 11 cases (92%) and WWTR1\u2010NUTM1<\/jats:italic> in 1 case (8%). These data corroborate findings from other recent investigations and further substantiate the utility of NUT IHC in diagnosing a subset of poroid neoplasms. In addition, two of our cases harbored fusions of YAP1<\/jats:italic> exon 6 to NUTM1<\/jats:italic> exon 3 and YAP1<\/jats:italic> exon 8 to NUTM1<\/jats:italic> exon 2, which have not been reported before in poroid neoplasms and indicate novel break points of YAP1<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.14265","type":"journal-article","created":{"date-parts":[[2022,6,4]],"date-time":"2022-06-04T07:44:17Z","timestamp":1654328657000},"page":"850-858","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Molecular analysis of NUT\u2010positive poromas and porocarcinomas identifies novel break points of YAP1::NUTM1<\/i> fusions"],"prefix":"10.1111","volume":"49","author":[{"given":"Justin T.","family":"Snow","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Dartmouth Hitchcock Medical Center Lebanon New Hampshire 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