{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:59:11Z","timestamp":1787313551690,"version":"build-2736575974"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,11,22]],"date-time":"2022-11-22T00:00:00Z","timestamp":1669075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100001170","name":"Hitchcock Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001170","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["5P30CA023108\u201037"],"award-info":[{"award-number":["5P30CA023108\u201037"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Melanocytic neoplasms can be challenging to diagnose. One well\u2010established diagnostic aid is the detection of copy number variation (CNV) in a few key genetic loci using conventional methods such as fluorescence in situ hybridization (FISH) and chromosomal microarray (CMA). Droplet digital polymerase chain reaction (ddPCR) is a novel, cost\u2010effective, rapid, and automated method to detect CNV.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We perform the first investigation of ddPCR to assay Ras\u2010responsive element\u2010binding protein\u20101 (\n                      <jats:italic>RREB1<\/jats:italic>\n                      ), the most common CNV in melanoma using formalin\u2010fixed, paraffin\u2010embedded (FFPE) melanocytic lesion samples; CMA data are used as the gold standard. Archival samples from 2013 to 2021 were analyzed, including 153 data points from 39 FFPE samples representing 34 patients. Benign, borderline, malignant, and metastatic melanocytic neoplasms were examined.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      ddPCR showed a sensitivity and specificity of 93.8% and 95.7% using one reference gene, and 87.5% and 100% using a different reference gene for\n                      <jats:italic>RREB1<\/jats:italic>\n                      gain detection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Here we show that ddPCR can provide inexpensive, rapid, and robust data on the commonest copy number alteration in melanoma. Future development and validation could provide a useful ancillary tool in the diagnosis of challenging melanocytic lesions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cup.14352","type":"journal-article","created":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T04:24:46Z","timestamp":1667449486000},"page":"169-177","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A novel method to assess copy number variation in melanoma: Droplet digital\n                    <scp>PCR<\/scp>\n                    for precise quantitation of the\n                    <scp>\n                      <i>RREB1<\/i>\n                    <\/scp>\n                    gene in formalin\u2010fixed, paraffin\u2010embedded melanocytic neoplasms, a\n                    <scp>proof\u2010of\u2010concept<\/scp>\n                    study"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1036-4601","authenticated-orcid":false,"given":"Keegan","family":"O'Hern","sequence":"first","affiliation":[{"name":"Dartmouth Geisel School of Medicine  Hanover New Hampshire USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachael","family":"Barney","sequence":"additional","affiliation":[{"name":"Clinical Genomics and Advanced Technology Laboratory Dartmouth\u2010Hitchcock Medical Center  Lebanon New Hampshire USA"},{"name":"Department of Pathology and Laboratory Medicine Dartmouth\u2010Hitchcock Medical Center  Lebanon New Hampshire USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meagan","family":"Chambers","sequence":"additional","affiliation":[{"name":"Dartmouth Geisel School of Medicine  Hanover New Hampshire USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Baker","sequence":"additional","affiliation":[{"name":"Dartmouth Geisel School of Medicine  Hanover New Hampshire USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirjana","family":"Stevanovic","sequence":"additional","affiliation":[{"name":"Dartmouth Geisel School of Medicine  Hanover New Hampshire USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory J.","family":"Tsongalis","sequence":"additional","affiliation":[{"name":"Clinical Genomics and Advanced Technology Laboratory Dartmouth\u2010Hitchcock Medical Center  Lebanon New Hampshire USA"},{"name":"Department of Pathology and Laboratory Medicine Dartmouth\u2010Hitchcock Medical Center  Lebanon New Hampshire USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward","family":"Hughes","sequence":"additional","affiliation":[{"name":"Clinical Genomics and Advanced Technology Laboratory Dartmouth\u2010Hitchcock Medical Center  Lebanon New Hampshire USA"},{"name":"Department of Pathology and Laboratory Medicine Dartmouth\u2010Hitchcock Medical Center  Lebanon New Hampshire USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aravindhan","family":"Sriharan","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Dartmouth\u2010Hitchcock Medical Center  Lebanon New Hampshire USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,11,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21654"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1502583"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1535\u20106108(03)00189\u20102"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002\u20109440(10)64393\u20103"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201053636\u2010x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379\u2010020\u20100499\u2010y"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1529\u20108019.2005.00055.x"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002\u20109440(10)63536\u20105"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u201062703\u2010727\u20103_12"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118488935"},{"key":"e_1_2_10_12_1","unstructured":"Mayo Clinical Laboratories.Clinical \u2013 Melanoma FISH. Accessed April 2021.https:\/\/www.mayocliniclabs.com\/test\u2010notifications\/attachment.php?id=66054."},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2015.02.1140"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/cup.12705"},{"key":"e_1_2_10_15_1","unstructured":"University of Michigan Laboratories.Chromosomal microarray analysis for melanoma. Accessed April 2021.https:\/\/mlabs.umich.edu\/tests\/chromosomal-microarray-analysis-melanoma"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1772678"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2012.197855"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0146784"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885\u2010019\u20105306\u20100"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2017.11.009"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s41241\u2010016\u20100007\u20103"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2015.11.027"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.40834"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.23079"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/cup.13057"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206257"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.16.10.5335"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1001\/archdermatol.2009.386"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2013.04.001"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathol.2016.11.008"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1158\/1538\u20107445.AM2021\u2010565"}],"container-title":["Journal of Cutaneous Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cup.14352","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cup.14352","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cup.14352","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T01:55:02Z","timestamp":1692496502000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cup.14352"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,22]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,2]]}},"alternative-id":["10.1111\/cup.14352"],"URL":"https:\/\/doi.org\/10.1111\/cup.14352","archive":["Portico"],"relation":{},"ISSN":["0303-6987","1600-0560"],"issn-type":[{"value":"0303-6987","type":"print"},{"value":"1600-0560","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,22]]},"assertion":[{"value":"2022-08-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}