{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T15:25:33Z","timestamp":1785684333017,"version":"3.56.0"},"reference-count":15,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T00:00:00Z","timestamp":1671580800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Melanocytic nevi existing in lymph nodes create a diagnostic challenge by mimicking metastases. PReferentially expressed Antigen in MElanoma (PRAME) immunohistochemical (IHC) stain can differentiate one from another. FLI\u20101 IHC expression has been shown in malignant melanoma with variable sensitivity while melanocytic nevi were reported to be negative. We hypothesized that FLI\u20101\/Melan\u2010A dual IHC staining may be used in the distinction of metastatic melanoma from nodal nevi and can be an alternative and\/or complementary to PRAME. In this study, we examined 13 lymph nodes with metastatic melanoma and 13 lymph nodes with benign deposits. We stained all of the lymph nodes with FLI\u20101, FLI\u20101\/Melan\u2010A dual, and PRAME IHC stains. In addition, we stained paired skin samples of the metastatic lymph nodes with FLI\u20101 and PRAME. In primary cutaneous melanomas, 11 of 13 were positive for FLI\u20101 and PRAME expression (85%). Malignant cells in 12 and 13 lymph nodes showed positive expression of PRAME and FLI\u20101, respectively. Only one case with a nevic cell deposit was weakly positive for FLI\u20101 and the remaining benign cases were negative for both FLI\u20101 and PRAME. Our results show that FLI\u20101\/Melan\u2010A dual stain is as sensitive and specific as PRAME in distinguishing lymph nodes with metastatic melanoma from nodal nevi. Further studies with larger case numbers are needed to support our significant results.<\/jats:p>","DOI":"10.1111\/cup.14373","type":"journal-article","created":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T18:10:21Z","timestamp":1669918221000},"page":"247-258","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<scp>FLI<\/scp>\u20101\/<scp>Melan\u2010A<\/scp> dual stain is an alternative to <scp>PRAME<\/scp> in differentiating metastatic melanoma from nodal nevus: A monocentric retrospective study"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2908-9128","authenticated-orcid":false,"given":"Hatice B.","family":"Zengin","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Rochester Medical Center  Rochester New York 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