{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:57:32Z","timestamp":1747184252760,"version":"3.40.5"},"reference-count":8,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T00:00:00Z","timestamp":1684454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2023,12]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>Access to vertical and transverse sections of a punch biopsy specimen improves the diagnosis of alopecia. Both two biopsy specimen and single\u2010punch biopsy specimen techniques to visualize both transverse and vertical sections have been described. Their comparative diagnostic certainty is not known. We aimed to assess the diagnostic certainty of a modified HoVert (mHoVert) method, without direct immunofluorescence (DIF), compared to the St John's protocol, a two\u2010biopsy technique with DIF.<\/jats:p><\/jats:sec>Methods<\/jats:title>Fifty\u2010seven cases of alopecia processed using the St John's protocol and 60 cases of alopecia processed using mHoVert were reviewed. Diagnoses made were rated as certain\/probable, possible, or uncertain, depending on the language in the histopathology report. Cases processed by the St John's protocol had final diagnosis and DIF result recorded.<\/jats:p><\/jats:sec>Results<\/jats:title>In the mHoVert group, significantly more diagnoses were certain\/probable (66%, 95% confidence interval [CI]: 57%\u201375%), compared to 46% (95% CI: 36%\u201356%) of diagnoses in the St John's protocol group (p<\/jats:italic>\u2009=\u20090.005). DIF result did not affect the final diagnosis in any of the 57 cases reviewed.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>DIF is not required in the diagnosis of most cases of alopecia. The mHoVert method provides more certain\/probable diagnoses than the St John's protocol and can reduce cost and patient morbidity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.14447","type":"journal-article","created":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T08:10:12Z","timestamp":1684483812000},"page":"1099-1103","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The modified HoVert<\/scp> (mHoVert<\/scp>) method improves diagnostic certainty compared to the St John's protocol for alopecia biopsy specimens: A retrospective single center study"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4824-7195","authenticated-orcid":false,"given":"Laura","family":"Adams","sequence":"first","affiliation":[{"name":"Royal Free Hospital London UK"},{"name":"Current institution: University Hospitals of Southampton Southampton UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Amphlett","sequence":"additional","affiliation":[{"name":"Royal Free Hospital London UK"},{"name":"Current institution: St George's University Hospitals Foundation Trust London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma","family":"Gardette","sequence":"additional","affiliation":[{"name":"Royal Free Hospital London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florence","family":"Deroide","sequence":"additional","affiliation":[{"name":"Royal Free Hospital London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Jones","sequence":"additional","affiliation":[{"name":"Royal Free Hospital London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,5,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0190\u20109622(95)90068\u20103"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/cup.12075"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000086436"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100560.2010.01669.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.4103\/jlp.jlp_4_17"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dsi.2017.08.008"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102230.2011.04154.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijt.ijt_66_17"}],"container-title":["Journal of Cutaneous Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cup.14447","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,9]],"date-time":"2023-11-09T09:30:44Z","timestamp":1699522244000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cup.14447"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,19]]},"references-count":8,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["10.1111\/cup.14447"],"URL":"https:\/\/doi.org\/10.1111\/cup.14447","archive":["Portico"],"relation":{},"ISSN":["0303-6987","1600-0560"],"issn-type":[{"type":"print","value":"0303-6987"},{"type":"electronic","value":"1600-0560"}],"subject":[],"published":{"date-parts":[[2023,5,19]]},"assertion":[{"value":"2022-06-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}