{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T16:31:16Z","timestamp":1748968276964,"version":"3.40.5"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T00:00:00Z","timestamp":1684972800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The clinicopathologic and genetic features of cutaneous melanoma with a <jats:italic>BRAF<\/jats:italic> V600K mutation are not well\u2010known. We aimed to evaluate these characteristics in comparison with those associated with <jats:italic>BRAF<\/jats:italic> V600E.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Real\u2010time polymerase chain reaction (PCR) and\/or the MassARRAY\u00ae system were used to detect <jats:italic>BRAF<\/jats:italic> V600K in 16 invasive melanomas and confirm <jats:italic>BRAF<\/jats:italic> V600E in another 60 cases. Immunohistochemistry and panel next\u2010generation sequencing were used to evaluate protein expression and tumor mutation burden, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The median age of melanoma patients harboring the <jats:italic>BRAF<\/jats:italic> V600K mutation (72.5\u2009years) was higher than those with the <jats:italic>BRAF<\/jats:italic> V600E (58.5\u2009years). The two groups also differed in sex (13\/16 [81.3%] male in the V600K group vs. 23\/60 [38.3%] in V600E) and in the frequency of scalp involvement (8\/16 [50.0%] in V600K vs. 1\/60 [1.6%] in V600E). The clinical appearance was similar to a superficial spreading melanoma. Histopathologically, non\u2010nested lentiginous intraepidermal spread and subtle solar elastosis were observed. One patient (1\/13, 7.7%) had a pre\u2010existing intradermal nevus. Diffuse PRAME immunoexpression was seen in only one (14.3%) of seven tested cases. Loss of p16 expression was observed in all 12 cases (100%) analyzed. The tumor mutation burden was 8 and 6 mutations\/Mb in the two tested cases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Melanoma carrying the <jats:italic>BRAF<\/jats:italic> V600K mutation showed the predominance on the scalp of elderly men, lentiginous intraepidermal growth, subtle solar elastosis, possible existence of intradermal nevus component, frequent loss of p16 immunoexpression, limited immunoreactivity for PRAME, and intermediate tumor mutation burden.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.14470","type":"journal-article","created":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T09:20:47Z","timestamp":1685006447000},"page":"739-747","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Clinicopathologic and genetic characterization of invasive melanoma with <i>BRAF<\/i><scp>V600K<\/scp> mutation: A study of 16 cases"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4165-1809","authenticated-orcid":false,"given":"Keisuke","family":"Goto","sequence":"first","affiliation":[{"name":"Department of Diagnostic Pathology Shizuoka Cancer Center Hospital  Sunto Japan"},{"name":"Department of Dermatology Hyogo Cancer Center  Akashi Japan"},{"name":"Department of Diagnostic Pathology and Cytology Osaka International Cancer Institute  Osaka Japan"},{"name":"Department of Pathology Tokyo Metropolitan Cancer and Infectious Disease Center Komagome Hospital  Tokyo Japan"},{"name":"Department of Pathology Itabashi Central Clinical Laboratory  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shusuke","family":"Yoshikawa","sequence":"additional","affiliation":[{"name":"Department of Dermatology Shizuoka Cancer Center Hospital  Sunto 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