{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:09:21Z","timestamp":1790788161417,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,7,2]],"date-time":"2023-07-02T00:00:00Z","timestamp":1688256000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Viral infection is an oncogenic factor in many hematolymphoid malignancies. We sought to determine the diagnostic yield of aligning off\u2010target reads incidentally obtained during targeted hematolymphoid next\u2010generation sequencing to a large database of viral genomes to screen for viral sequences within tumor specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Alignment of off\u2010target reads to viral genomes was performed using magicBLAST. Localization of Merkel cell polyomavirus (MCPyV) RNA was confirmed by RNAScope in situ hybridization. Integration analysis was performed using Virus\u2010Clip.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Four cases of post\u2010cardiac\u2010transplant folliculotropic mycosis fungoides (fMF) and one case of peripheral T\u2010cell lymphoma (PTCL) were positive in off\u2010target reads for MCPyV DNA. Two of the four cases of posttransplant fMF and the case of PTCL showed localization of MCPyV RNA to malignant lymphocytes, whereas the remaining two cases of posttransplant fMF showed MCPyV RNA in keratinocytes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings raise the question of whether MCPyV may play a role in rare cases of T\u2010lymphoproliferative disorders, particularly in the skin and in the heavily immunosuppressed posttransplant setting.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.14486","type":"journal-article","created":{"date-parts":[[2023,7,3]],"date-time":"2023-07-03T05:39:27Z","timestamp":1688362767000},"page":"835-844","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Identification and confirmation via <scp>in situ<\/scp> hybridization of Merkel cell polyomavirus in rare cases of posttransplant cutaneous T\u2010cell 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