{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T18:33:18Z","timestamp":1783103598194,"version":"3.54.6"},"reference-count":23,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,7,3]],"date-time":"2023-07-03T00:00:00Z","timestamp":1688342400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005190","name":"Melanoma Research Alliance","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005190","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Cancer Institute"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>TERT<\/jats:italic> gene amplification (TGA) is a mechanism of telomerase reverse transcriptase (TERT) upregulation frequently utilized by acral melanomas (AMs). Currently, the utility of TERT immunohistochemistry (IHC) to predict TGA status in AMs is poorly documented.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>AMs (26 primary and 3 metastatic) and non\u2010acral cutaneous melanomas (6 primary) were subjected to immunohistochemical analysis using anti\u2010TERT antibody to demonstrate protein expression and fluorescence in situ hybridization (FISH) to assess genomic copy number alteration. The relationship between TERT immunoreactivity and TGA confirmed by FISH was assessed using logistic regression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>TERT expression was seen in 50% (13\/26) of primary and 100% (3\/3) of metastatic AMs and 50% (3\/6) of primary non\u2010acral cutaneous melanomas. TGA was found in 15% (4\/26) and 67% (2\/3) of primary and metastatic AMs and 17% (1\/6) of non\u2010acral cutaneous melanomas. The intensity of TERT immunoreactivity correlated with TGA (<jats:italic>p<\/jats:italic>\u2009=\u20090.04) and a higher <jats:italic>TERT<\/jats:italic> copy number\u2010to\u2010control ratio in AMs, with a correlation coefficient of 0.41 (<jats:italic>p<\/jats:italic>\u2009=\u20090.03). The sensitivity and specificity of TERT immunoreactivity for predicting TGA in AMs were 100% and 57%, with corresponding positive and negative predictive values of 38% and 100%, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The clinical utility of TERT IHC to predict TGA status in AMs appears to be limited given its low specificity and positive predictive value.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.14494","type":"journal-article","created":{"date-parts":[[2023,7,4]],"date-time":"2023-07-04T01:00:16Z","timestamp":1688432416000},"page":"845-851","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Telomerase reverse transcriptase immunohistochemical expression is sensitive but not specific for <i>TERT<\/i> gene amplification in acral melanoma"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5867-1403","authenticated-orcid":false,"given":"Woo Cheal","family":"Cho","sequence":"first","affiliation":[{"name":"Department of Pathology The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Clinical and Translational Sciences, Department of Internal Medicine The University of Texas McGovern Medical School at Houston  Houston Texas USA"},{"name":"Biostatistics\/Epidemiology\/Research Design (BERD) Component, Center for Clinical and Translational Sciences (CCTS) The University of Texas Health Science Center at Houston  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Gu","sequence":"additional","affiliation":[{"name":"Cytogenetics Training Laboratory, School of Health Professions The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei\u2010Lien","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology The University of Texas MD Anderson Cancer Center  Houston Texas USA"},{"name":"Department of Translational Molecular Pathology The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Ning","sequence":"additional","affiliation":[{"name":"Department of Biostatistics The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Sfamenos","sequence":"additional","affiliation":[{"name":"Cytogenetics Training Laboratory, School of Health Professions The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4576-0049","authenticated-orcid":false,"given":"Pavandeep","family":"Gill","sequence":"additional","affiliation":[{"name":"Department of Pathology Royal Jubilee Hospital  Victoria British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5346-2669","authenticated-orcid":false,"given":"Priyadharsini","family":"Nagarajan","sequence":"additional","affiliation":[{"name":"Department of Pathology The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0630-6222","authenticated-orcid":false,"given":"Jonathan L.","family":"Curry","sequence":"additional","affiliation":[{"name":"Department of Pathology The University of Texas MD Anderson Cancer Center  Houston Texas USA"},{"name":"Department of Translational Molecular Pathology The University of Texas MD Anderson Cancer Center  Houston Texas USA"},{"name":"Department of Dermatology The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander J.","family":"Lazar","sequence":"additional","affiliation":[{"name":"Department of Pathology The University of Texas MD Anderson Cancer Center  Houston Texas USA"},{"name":"Department of Translational Molecular Pathology The University of Texas MD Anderson Cancer Center  Houston Texas USA"},{"name":"Department of Dermatology The University of Texas MD Anderson Cancer Center  Houston Texas USA"},{"name":"Department of Genomic Medicine The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9204-7161","authenticated-orcid":false,"given":"Victor G.","family":"Prieto","sequence":"additional","affiliation":[{"name":"Department of Pathology The University of Texas MD Anderson Cancer Center  Houston Texas USA"},{"name":"Department of Dermatology The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7422-0661","authenticated-orcid":false,"given":"Carlos A.","family":"Torres\u2010Cabala","sequence":"additional","affiliation":[{"name":"Department of Pathology The University of Texas MD Anderson Cancer Center  Houston Texas USA"},{"name":"Department of Dermatology The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3398-0573","authenticated-orcid":false,"given":"Phyu P.","family":"Aung","sequence":"additional","affiliation":[{"name":"Department of Pathology The University of Texas MD Anderson Cancer Center  Houston Texas 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