{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T06:37:35Z","timestamp":1767163055656,"version":"build-2238731810"},"reference-count":14,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T00:00:00Z","timestamp":1692144000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Pemphigus is a chronic potentially fatal autoimmune bullous disorder. Pemphigus vulgaris (PV) and pemphigus foliaceus (PF) are the two common subtypes. PV is the most common and aggressive type characterized by oral mucosal erosions and cutaneous lesions. PF presents with blisters on the scalp, face, and upper trunk, and spares the mucosae. Direct immunofluorescence (DIF) is the gold standard for diagnosis. Immunohistochemistry (IHC) is an emerging alternate diagnostic tool. In this study, our objectives were to identify the staining patterns of desmoglein 1 (dsg 1) and desmoglein 3 (dsg 3) IHC and to correlate the same with autoantibody levels and clinical severity in patients with PV and PF.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Forty\u2010nine clinically, histologically, and DIF\u2010confirmed cases of pemphigus were included in the study. The IHC patterns were scored from 0 to 3+ with 3+ dsg 1 IHC exhibiting intense membranous staining in the upper layers of the epidermis and 3+ dsg 3 IHC showing intense basal layer staining. Enzyme\u2010linked immunosorbent assay (ELISA) for anti\u2010dsg 1 and 3 antibodies was performed in 38 cases where serum samples were available. The pemphigus disease activity index system was utilized for clinical scoring.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A 0 to 1+ score was observed for dsg 1 IHC in 100% of PF cases. A score of 0 to 1+ was observed for dsg 3 IHC in 97.3% of PV cases. One hundred percent of cases with PF and 83.9% of patients with PV tested positive for ELISA anti\u2010dsg 1 and 3 antibody titers, respectively. Anti\u2010dsg 1 and 3 ELISA titers significantly correlated with the dsg 1 and dsg 3 IHC scores. The mucosal scores showed a significant association with both dsg 1 and 3 IHC (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). The cutaneous scores showed a significant association with the dsg 3 IHC (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The IHC patterns for dsg 1 and 3 proved reliable in giving concordant results with the ELISA antibody titers and clinical severity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cup.14501","type":"journal-article","created":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T12:09:47Z","timestamp":1692187787000},"page":"1104-1109","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Correlation of desmoglein 1 and 3 immunohistochemistry with autoantibody levels and clinical severity in pemphigus"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2375-4732","authenticated-orcid":false,"given":"Aiswarya","family":"Pradeep","sequence":"first","affiliation":[{"name":"Department of Pathology Amrita Institute of Medical Sciences  Kochi Kerala India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malini","family":"Eapen","sequence":"additional","affiliation":[{"name":"Department of Pathology Amrita Institute of Medical Sciences  Kochi Kerala India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soumya","family":"Jagadeeshan","sequence":"additional","affiliation":[{"name":"Department of Dermatology Amrita Institute of Medical Sciences  Kochi Kerala India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keerthiga","family":"Kani","sequence":"additional","affiliation":[{"name":"Department of Dermatology Amrita Institute of Medical Sciences  Kochi Kerala India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,8,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973\u2010029X.190979"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1590\/abd1806\u20104841.20199011"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/0019\u20105154.80403"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.det.2011.03.014"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.4103\/0019\u20105154.135488"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijd.IJD_595_16"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20104362.1993.tb02785"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403\u2010005\u20100573\u20109"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-029X.40935"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.7241\/ourd.20134.148"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063870201400110"},{"issue":"11","key":"e_1_2_8_13_1","first-page":"1663","article-title":"Evaluation of sensitivity and specificity of enzyme\u2010linked immunosorbent assay (ELISA) for detecting antidesmoglein 1 and 3 in Thai patients with pemphigus vulgaris and foliaceus","volume":"91","author":"Kulkollakarn S","year":"2008","journal-title":"J Med Assoc Thai"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000008801"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523\u20101747.1998.00107"}],"updated-by":[{"DOI":"10.1111\/cup.14582","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2023,12,22]],"date-time":"2023-12-22T00:00:00Z","timestamp":1703203200000}}],"container-title":["Journal of Cutaneous Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cup.14501","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,9]],"date-time":"2023-11-09T04:30:31Z","timestamp":1699504231000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cup.14501"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,16]]},"references-count":14,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["10.1111\/cup.14501"],"URL":"https:\/\/doi.org\/10.1111\/cup.14501","archive":["Portico"],"relation":{},"ISSN":["0303-6987","1600-0560"],"issn-type":[{"value":"0303-6987","type":"print"},{"value":"1600-0560","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,16]]},"assertion":[{"value":"2022-09-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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