{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:08:20Z","timestamp":1787875700047,"version":"build-2784847793"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T00:00:00Z","timestamp":1699228800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Diagnosis of early mycosis fungoides (eMF) is challenging and often delayed as many of its clinical and histopathologic features may mimic various benign inflammatory dermatoses (BIDs). The products of the thymocyte selection\u2010associated high mobility group box (<jats:italic>TOX<\/jats:italic>), twist family BHLH transcription factor 1 (<jats:italic>TWIST1<\/jats:italic>), signal transducer and activator of transcription 4 (<jats:italic>STAT4<\/jats:italic>), and special AT\u2010rich sequence\u2010binding protein 1 (<jats:italic>SATB1<\/jats:italic>) genes function as transcription factors and are involved in the pathogenesis of MF.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>We aim to determine the diagnostic value of TOX, TWIST1, STAT4, and SATB1 protein expressions in eMF.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This non\u2010randomized, controlled, prospective analytic study was conducted by performing immunohistochemistry staining with TOX, TWIST1, STAT4, and SATB1 polyclonal antibodies in lesional skin biopsies of eMF and BID patients. Nuclear staining of lymphocytes was compared between eMF and BIDs, and the capacity of these antibodies to predict eMF was determined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immunostainings with anti\u2010TWIST1 showed an increase in protein expression (<jats:italic>p<\/jats:italic>\u2009=\u20090.003) and showed a decrease with anti\u2010SATB1 antibodies in eMF compared to BIDs (<jats:italic>p<\/jats:italic>\u2009=\u20090.005) while anti\u2010TOX and anti\u2010STAT4 antibodies did not exhibit significant differences (<jats:italic>p<\/jats:italic>\u2009=\u20090.384; <jats:italic>p<\/jats:italic>\u2009=\u20090.150). Receiver operating characteristic analysis showed that immunohistochemical evaluations of TWIST1 and SATB1 protein expressions can differentiate eMF (area under the curve [AUC]: 0.728, 95% confidence interval [CI]: 0.605\u20130.851, <jats:italic>p<\/jats:italic>\u2009=\u20090.002; AUC: 0.686, 95% CI: 0.565\u20130.807, <jats:italic>p<\/jats:italic>\u2009=\u20090.013).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>TWIST1 and SATB1 are potential diagnostic markers for the histologic diagnosis of eMF.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.14557","type":"journal-article","created":{"date-parts":[[2023,11,7]],"date-time":"2023-11-07T05:25:08Z","timestamp":1699334708000},"page":"232-238","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<scp>TOX<\/scp>, <scp>TWIST1<\/scp>, <scp>STAT4<\/scp>, and <scp>SATB1<\/scp> protein expressions in early\u2010stage mycosis fungoides"],"prefix":"10.1111","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1536-6035","authenticated-orcid":false,"given":"Sinem","family":"\u00d6rnek","sequence":"first","affiliation":[{"name":"Department of Dermatology Health Sciences University Diskapi Yildirim Beyazit Training and Research Hospital  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selver","family":"Ozekinci","sequence":"additional","affiliation":[{"name":"Department of Pathology Dicle University Hospital  Diyarbakir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tugba","family":"Ipin","sequence":"additional","affiliation":[{"name":"Department of Pathology Cemil Tascioglu City Hospital  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emek","family":"Kocaturk","sequence":"additional","affiliation":[{"name":"Department of Dermatology Koc University School of Medicine  Istanbul Turkey"},{"name":"Institute of Allergy, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin Freie Universit\u00e4t Berlin and Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2018.09.001"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijwd.2016.11.006"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478\u2010199407000\u201000001"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.pas.0000153121.57515.c6"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478\u2010200001000\u201000005"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2013.07.049"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2007.08.004"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2005.08.057"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102559.2006.02406.x"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12578"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13261"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2012.13"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.2031"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0117479"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403\u2010014\u20101501\u20107"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2013.461"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201004\u20101253"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100560.2012.01883.x"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102002\u201011\u20103574"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102005\u201007\u20102813"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102013\u201010\u2010534693"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102010\u201007\u2010294819"},{"issue":"2","key":"e_1_2_11_24_1","first-page":"101","article-title":"Correlation of SATB1 expression with clinical course of cutaneous T\u2010cell lymphomas","volume":"63","author":"Grzanka A","year":"2012","journal-title":"Pol J Pathol"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2014.3597"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2018.03.1526"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni767"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/15.8.3439"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.17.2207"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.4161\/15384101.2014.965061"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.8.4388"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092\u20108674(92)90432\u2010c"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.14.5.521"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102007\u201003\u2010055749"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.17258"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1590\/abd1806\u20104841.20187106"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555\u20101971"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.15000"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(96)00727-5"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0092\u20108674(03)00002\u20103"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2007.08.003"},{"issue":"1","key":"e_1_2_11_42_1","first-page":"189","article-title":"Expression of SATB1, MTI\/II and Ki\u201067 in mycosis fungoides","volume":"36","author":"Jankowska\u2010Konsur A","year":"2016","journal-title":"Anticancer Res"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2020.05.120"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102014\u201005\u2010571778"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.14508"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics12010220"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics12071582"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.8.5.409"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.4161\/15384101.2014.947759"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajd.13420"}],"container-title":["Journal of Cutaneous Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cup.14557","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T08:17:58Z","timestamp":1706775478000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cup.14557"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,6]]},"references-count":49,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.1111\/cup.14557"],"URL":"https:\/\/doi.org\/10.1111\/cup.14557","archive":["Portico"],"relation":{},"ISSN":["0303-6987","1600-0560"],"issn-type":[{"value":"0303-6987","type":"print"},{"value":"1600-0560","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,6]]},"assertion":[{"value":"2023-02-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}