{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T15:33:14Z","timestamp":1769268794639,"version":"3.49.0"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,2,5]],"date-time":"2024-02-05T00:00:00Z","timestamp":1707091200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Histopathologic overlap between cutaneous squamous cell carcinoma (cSCC) and its indolent mimics likely leads to the overdiagnosis of cSCC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To perform a pilot study of the p53 immunohistochemical scoring system developed on vulvar squamous lesions in cSCC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The consistency and reliability of p53 immunostaining using a scoring system developed on vulvar cases, as compared with <jats:italic>TP53<\/jats:italic> genomic sequencing, was studied in an initial cohort of 28 cutaneous cases. p53 labeling was further assessed in an additional 63 cases of atypical squamous lesions, including 20 atypical squamous lesions classified by the authors as benign, 22 cases diagnosed as cSCC without high\u2010risk features, and 21 cases of high\u2010risk cSCC (cSCC\u2010HR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The concordance of p53 labeling and <jats:italic>TP53<\/jats:italic> sequencing was 82.1%. Four positive patterns of p53 mutation were identified: basal, parabasal\/diffuse, null, and cytoplasmic. p53 positivity in atypical, benign squamous lesions (10%) was significantly lower than that of low\u2010risk cSCC (63.6%, <jats:italic>p<\/jats:italic>\u2009=\u20090.0004) or cSCC\u2010HR (90.5%, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). p53 positivity in low\u2010risk cSCC versus cSCC\u2010HR was not statistically significant (<jats:italic>p<\/jats:italic>\u2009=\u20090.07).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>p53 Labeling may be a helpful biomarker to support the diagnosis of cSCC and distinguish cSCC from atypical but benign mimics.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.14591","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T07:37:29Z","timestamp":1707291449000},"page":"379-386","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A pilot study of p53 immunohistochemistry in atypical squamous lesions, using a vulvar scoring system"],"prefix":"10.1111","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1016-7851","authenticated-orcid":false,"given":"Na","family":"Niu","sequence":"first","affiliation":[{"name":"Department of Pathology Yale School of Medicine  New Haven Connecticut USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon F.","family":"Roy","sequence":"additional","affiliation":[{"name":"Department of Dermatology Yale School of Medicine  New Haven Connecticut USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2270-2524","authenticated-orcid":false,"given":"Christine J.","family":"Ko","sequence":"additional","affiliation":[{"name":"Department of Pathology Yale School of Medicine  New Haven Connecticut USA"},{"name":"Department of Dermatology Yale School of Medicine  New Haven Connecticut USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,2,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathol.2022.12.345"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12601"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41525\u2010021\u201000226\u20104"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2017.04.1119"},{"issue":"3","key":"e_1_2_9_6_1","first-page":"591","article-title":"Melanomas of 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