{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T14:46:50Z","timestamp":1787928410145,"version":"build-2784847793"},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,6,21]],"date-time":"2024-06-21T00:00:00Z","timestamp":1718928000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>PReferentially expressed Antigen in MElanoma (PRAME) has shown utility in differentiating benign from malignant melanocytic neoplasms. In this study, we investigated the clinical significance of PRAME expression in dysplastic nevi (DN) and nevus\u2010associated melanoma in situ (MIS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We included 172 DN and 38 nevus\u2010associated MIS from our institutional archive. PRAME positive expression was defined as nuclear staining in at least 75% of melanocytes. In addition, relevant studies from PubMed and Web of Science were incorporated into a meta\u2010analysis using the random\u2010effects model to assess PRAME expression in MIS and DN.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our institutional data revealed that 71.1% of nevus\u2010associated MIS cases exhibited positive PRAME expression in the MIS components, whereas all DN components were negative for PRAME. 5.7% of cases diagnosed as DN in our cohort demonstrated diffuse positivity for PRAME. Notably, MIS associated with DN displaying epidermal and dermal components displayed a higher likelihood of PRAME positivity compared to those arising on a background of DN with solely epidermal (junctional) components (84% vs. 46%, <jats:italic>p<\/jats:italic>\u2009=\u20090.024). The meta\u2010analysis indicated that the pooled PRAME positivity in MIS and DN was 54.5% and 1.9%, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>PRAME is a valuable immunohistochemical marker for differentiating MIS from DN, particularly in the context of nevus\u2010associated MIS.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.14671","type":"journal-article","created":{"date-parts":[[2024,6,21]],"date-time":"2024-06-21T09:38:35Z","timestamp":1718962715000},"page":"783-789","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Clinical implication of <scp>PRAME<\/scp> immunohistochemistry in differentiating melanoma in situ and dysplastic nevus in non\u2010acral nevus\u2010associated melanoma in situ: An institutional experience and meta\u2010analysis"],"prefix":"10.1111","volume":"51","author":[{"given":"Chau M.","family":"Bui","sequence":"first","affiliation":[{"name":"Department of Pathology University of Rochester Medical Center  Rochester New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huy Gia","family":"Vuong","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Iowa Hospitals and Clinics  Iowa City Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minh\u2010Khang","family":"Le","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Yamanashi  Chuo Yamanashi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josephine","family":"D'Angelo","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Rochester Medical Center  Rochester New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen","family":"Mannava","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Rochester Medical Center  Rochester New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7301-8274","authenticated-orcid":false,"given":"Bruce R.","family":"Smoller","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Rochester Medical Center  Rochester New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1074\u20107613(00)80426\u20104"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.2217\/bmm.12.65"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.CCR\u201017\u20102005"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.02.110"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.9977"},{"issue":"18","key":"e_1_2_10_7_1","first-page":"4090","article-title":"Heterogeneous 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