{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T17:58:14Z","timestamp":1781632694852,"version":"3.54.5"},"reference-count":16,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T00:00:00Z","timestamp":1734566400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2025,3]]},"abstract":"ABSTRACT<\/jats:title>Background<\/jats:title>Mpox (formerly known as monkeypox), a zoonotic disease caused by Monkeypox<\/jats:italic> virus (MPXV<\/jats:italic>), has become an international outbreak since May 2022. Mpox often presents with a mild systemic illness and a characteristic vesiculopustular skin eruption. In addition to molecular testing, histopathology of cutaneous lesions usually shows\u00a0distinctive findings, such as epidermal necrosis, balloon degeneration, papillary dermal edema, and focal dermal necrosis, which have proven helpful in the diagnosis of mpox. Viral cytopathic changes with areas of multinucleation, smudging of the nuclei, and intracytoplasmic inclusions have also been described. Although useful, these features are relatively nonspecific. The use of a monoclonal antibody for immunohistochemical (IHC) staining of MPXV<\/jats:italic> may be a useful tool in confirming mpox infection.<\/jats:p><\/jats:sec>Methods<\/jats:title>Three cases of PCR\u2010confirmed mpox were biopsied and subjected to IHC staining with a monoclonal MPXV<\/jats:italic>\u2010specific antibody targeting viral envelope protein A29. As controls, cell lines transduced to express other MPXV<\/jats:italic> viral antigens and samples of cutaneous viral infections involving Molluscum contagiosum<\/jats:italic>, Herpes simplex<\/jats:italic>, Herpes zoster<\/jats:italic>, or Cytomegalovirus<\/jats:italic> were also subjected to IHC staining with this antibody.<\/jats:p><\/jats:sec>Results<\/jats:title>All three mpox patient biopsies performed on lesional skin subjected to MPXV<\/jats:italic> IHC staining reliably detected viral infection in lesional skin with a diffuse cytoplasmic and focally nuclear staining pattern. No staining was seen in transduced cell lines expressing off\u2010target MPXV<\/jats:italic> viral antigens and in lesional skin of other common viral infections listed above.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The monoclonal MPXV<\/jats:italic>\u2010specific antibody may be used as an adjunct tool to confirm mpox infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cup.14776","type":"journal-article","created":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T10:10:03Z","timestamp":1734603003000},"page":"244-249","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Detecting Monkeypox<\/i><\/scp> Virus by Immunohistochemistry"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1251-4514","authenticated-orcid":false,"given":"Spencer","family":"Ng","sequence":"first","affiliation":[{"name":"Division of Dermatology, Department of Medicine, and Department of Pathology and Immunology Washington University School of Medicine St. Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0454-2635","authenticated-orcid":false,"given":"Carla","family":"Stephan","sequence":"additional","affiliation":[{"name":"Department of Pathology Weill Cornell Medical College New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mytrang","family":"Do","sequence":"additional","affiliation":[{"name":"Department of Dermatology Weill Cornell Medical College New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise","family":"Frosina","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Memorial Sloan Kettering Cancer Center New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Achim","family":"Jungbluth","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Memorial Sloan Kettering Cancer Center New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0888-9601","authenticated-orcid":false,"given":"Klaus J.","family":"Busam","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Memorial Sloan Kettering Cancer Center New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linglei","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Virginia Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,19]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"World Health Organization \u201c2022\u201023 Mpox (Monkeypox) Outbreak: Global Trends.\u201d (2023) 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