{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T19:39:56Z","timestamp":1771357196020,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,8,17]],"date-time":"2025-08-17T00:00:00Z","timestamp":1755388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Acanthamoeba<\/jats:italic> is a free\u2010living ameba, known to most commonly cause amebic keratitis in contact lens users and granulomatous amebic encephalitis in immunocompromised patients. Cutaneous Acanthamoebiasis as a single\u2010organ manifestation is less common. We report two cases seen in our department. Case one is a 23\u2010year\u2010old hospitalized patient with systemic lupus erythematosus and pancytopenia who acutely developed nodules on the extremities. Biopsy showed lymphohistiocytic inflammation and focal coagulative necrosis in the subcutaneous fat. Acanthamoeba trophozoites and cysts were apparent in H&amp;E\u2010stained sections and highlighted by PAS and GMS stains. PCR studies were positive for <jats:italic>Acanthamoeba<\/jats:italic> species. Case two is an 81\u2010year\u2010old with diffuse large B\u2010cell lymphoma on idelalisib, who presented with a three\u2010week history of ulcerating nodules on the extremities. Biopsy from the left arm exhibited a mixed deep dermal infiltrate with neutrophils and histiocytes while biopsy from the right arm exhibited granulomatous inflammation. Acanthamoeba trophozoites and cysts were apparent in H&amp;E and PAS\u2010stained sections, confirmed to be <jats:italic>Acanthamoeba<\/jats:italic> by PCR. Cutaneous Acanthamoebiasis should be considered in biopsies of cutaneous nodules from immunocompromised patients. The histopathologic inflammatory changes are variable and non\u2010specific, while the organisms are easily overlooked due to their resemblance to histiocytes. Familiarity with these features is essential for accurate, timely diagnosis.<\/jats:p>","DOI":"10.1111\/cup.14854","type":"journal-article","created":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T06:56:57Z","timestamp":1755500217000},"page":"681-685","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cutaneous Acanthamoebiasis: Two Cases Highlighting Diverse Histopathologic Findings"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1415-8071","authenticated-orcid":false,"given":"Nadia","family":"Siddiqui","sequence":"first","affiliation":[{"name":"University of Washington School of Medicine  Seattle Washington USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3373-5399","authenticated-orcid":false,"given":"Bicong","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Washington  Seattle Washington USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4791-7962","authenticated-orcid":false,"given":"Lindsay","family":"Gunnell","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Washington  Seattle Washington USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evan","family":"George","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Washington  Seattle Washington USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1984-9552","authenticated-orcid":false,"given":"Oliver","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Washington  Seattle Washington USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofac682"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/20499361241228340"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2004.06.004"},{"key":"e_1_2_9_5_1","unstructured":"CDC Free Living Amebic InfectionsJune 5 2024 accessed December 18 2024 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