{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T20:36:46Z","timestamp":1767818206346,"version":"3.49.0"},"reference-count":11,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T00:00:00Z","timestamp":1763942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2026,2]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n Previous studies suggest that autofluorescence of pathogenic fungi in hematoxylin\u2013eosin (HE) stained sections under ultraviolet (UV) light may offer rapid screening for fungal infections.<\/jats:p>\n <\/jats:sec>\n \n Aims<\/jats:title>\n To evaluate the diagnostic utility of autofluorescence in HE\u2010stained sections for cutaneous sporotrichosis and compare it with Periodic acid\u2010Schiff (PAS) and Gomori's methenamine silver (GMS) staining.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n A retrospective analysis included 49 fungal culture\u2010confirmed sporotrichosis cases and eight chromoblastomycosis controls from 2022 to 2024. HE\u2010, PAS\u2010, and GMS\u2010stained sections were examined. Autofluorescence in HE\u2010stained sections was assessed using fluorescent microscopy at UV light mode (340\u2013380\u2009nm).<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Most patients presented with fixed cutaneous lesions (65.31%,\n n<\/jats:italic>\n \u2009=\u200932), predominantly on the upper extremities (73.47%,\n n<\/jats:italic>\n \u2009=\u200936). Histologically, suppurative granuloma (55.10%,\n n<\/jats:italic>\n \u2009=\u200927) was the most common pattern. Autofluorescence revealed\n Sporothrix<\/jats:italic>\n spores with peripheral green\u2010yellow fluorescence against an olive\u2010yellow background, though erythrocyte interference limited detection in hemorrhagic areas. Only 12\/49 cases (24.5%) were autofluorescence\u2010positive, all of which were also PAS\u2010 or GMS\u2010positive. Sensitivity was significantly lower than PAS (34.7%,\n p<\/jats:italic>\n *\u2009=\u20090.063) and GMS (38.8%,\n p<\/jats:italic>\n *\u2009=\u20090.021). No false positive occurred in chromoblastomycosis controls. Interobserver agreement was near\u2010perfect (\u03ba\u2009=\u20090.91).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Autofluorescence enables rapid\n Sporothrix<\/jats:italic>\n detection with perfect specificity and excellent interobserver agreement, but its low sensitivity limits standalone use. PAS and GMS remain superior for definitive diagnosis. While its reagent\u2010free, cost\u2010effective nature supports adjunct screening where special stains are unavailable, erythrocyte interference and technical variability require protocol optimization.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/cup.70022","type":"journal-article","created":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T01:14:46Z","timestamp":1764033286000},"page":"193-198","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Diagnostic Value of Autofluorescence in Hematoxylin\u2013Eosin\u2010Stained Sections for Cutaneous Sporotrichosis"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8008-718X","authenticated-orcid":false,"given":"Xiaopo","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pathology, Hospital for Skin Diseases, Institute of Dermatology Chinese Academy of Medical Sciences and Peking Union Medical College Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuying","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Mycology, Hospital for Skin Diseases, Institute of Dermatology Chinese Academy of Medical Sciences and Peking Union Medical College Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2006.05.006"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.2010.01626.x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myz035"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/0377-4929.41690"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-1-21"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/cup.12086"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/79.5.587"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1067\/mjd.2002.120623"},{"key":"e_1_2_11_10_1","first-page":"93","article-title":"Autofluorescence of Eosinophilic Substances","volume":"110","author":"Huntington H.","year":"1986","journal-title":"Archives of Pathology & Laboratory Medicine"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.13588"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2009.10.004"}],"container-title":["Journal of Cutaneous Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cup.70022","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T19:25:53Z","timestamp":1767813953000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cup.70022"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,24]]},"references-count":11,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1111\/cup.70022"],"URL":"https:\/\/doi.org\/10.1111\/cup.70022","archive":["Portico"],"relation":{},"ISSN":["0303-6987","1600-0560"],"issn-type":[{"value":"0303-6987","type":"print"},{"value":"1600-0560","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,24]]},"assertion":[{"value":"2025-03-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}