{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T06:38:02Z","timestamp":1792132682591,"version":"4.4.0"},"reference-count":16,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T00:00:00Z","timestamp":1766448000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T00:00:00Z","timestamp":1766448000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Next\u2010generation sequencing (NGS) is becoming more commonly used for diagnosis in dermatopathology. It's critical to appraise its efficacy and limitations. Distinguishing benign deep penetrating nevi (DPN) from deep penetrating like\u2010melanoma (DPN\u2010M) is a challenging diagnostic scenario even for experienced dermatopathologists.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We sent a two\u2010phase survey (pre\u2010and postgenomics) to 32 experienced dermatopathologists to evaluate 39 diagnostically challenging cases from the DPN\/WNT\u2010activated family of melanocytic neoplasms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      With NGS data, interobserver agreement improved from 0.41 to 0.51 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001) in distinguishing DPN\u2010M from nonmelanoma cases. Overall diagnostic accuracy improved, mostly driven by a 16% increase in accurate diagnosis of DPN\u2010M. However, in two cases, the inclusion of genomics shifted the majority vote from a correct to an incorrect diagnosis. A total of 218 diagnostic changes occurred between Survey 1 and 2. Among the changes, 132 votes moved toward the correct diagnosis while 86 moved toward an incorrect diagnosis. The shift in voting which resulted in improved diagnostic accuracy was statistically significant (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>NGS has the potential to improve interobserver agreement and diagnostic accuracy. We provide guidance on the utilization of bioinformatic data to maximize its benefits and improve diagnostic accuracy and interobserver agreement.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cup.70049","type":"journal-article","created":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T03:51:27Z","timestamp":1766548287000},"page":"293-301","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The Impact of Next\u2010Generation Sequencing on Interobserver Agreement and Diagnostic Accuracy of Deep Penetrating Melanocytic Neoplasms"],"prefix":"10.1111","volume":"53","author":[{"given":"Julia Edwin","family":"Jeyakumar","sequence":"first","affiliation":[{"name":"Department of Dermatology Feinberg School of Medicine, Northwestern University  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Afua Konadu","family":"Addo","sequence":"additional","affiliation":[{"name":"Department of Dermatology Feinberg School of Medicine, Northwestern University  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haya Mary","family":"Beydoun","sequence":"additional","affiliation":[{"name":"Department of Dermatology Feinberg School of Medicine, Northwestern University  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shantel","family":"Olivares","sequence":"additional","affiliation":[{"name":"Department of Dermatology Feinberg School of Medicine, Northwestern University  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8515-2649","authenticated-orcid":false,"given":"Armita","family":"Bahrami","sequence":"additional","affiliation":[{"name":"Department of Pathology Emory University School of Medicine  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiagarajah","family":"Balamurugan","sequence":"additional","affiliation":[{"name":"Department of Pathology Royal Surrey NHS Foundation Trust  Guildford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8086-8544","authenticated-orcid":false,"given":"Raymond L.","family":"Barnhill","sequence":"additional","affiliation":[{"name":"Department of Translational Research Institut Curie, Paris Sciences and Lettres Research University, and UFR of Medicine, University of Paris Cit\u00e9  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willeke A. 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