{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:01:34Z","timestamp":1792119694013,"version":"4.4.0"},"reference-count":23,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Malignant melanoma (MM) and proliferative nodules (PN) may arise in large\/giant congenital melanocytic nevi (LCMN\/GCMN), posing clinical and pathological challenges in differential diagnosis. We investigated immunohistochemical expression of PRAME and H3K27me3 in MMs and PNs in LCMN\/GCMN, examining their potential utility.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Three MMs and six PNs arising in LCMN\/GCMN were subjected to immunohistochemical analyses. PRAME staining was scored as percentage of positive cells and H3K27me3 as follows: score0\u2009=\u2009no staining; score1\u2009=\u20091%\u201325%; score2\u2009=\u200926%\u201350%; score3\u2009=\u200951%\u201375%; score4\u2009=\u200976%\u2013100% staining in tumor cells.\n                      <jats:italic>BRAF<\/jats:italic>\n                      ,\n                      <jats:italic>NRAS<\/jats:italic>\n                      ,\n                      <jats:italic>KIT<\/jats:italic>\n                      ,\n                      <jats:italic>GNAQ, GNA11<\/jats:italic>\n                      , and\n                      <jats:italic>TERT<\/jats:italic>\n                      mutations were tested by polymerase chain reaction\u2010based direct Sanger sequencing for 3\u2009MMs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Mean age was 6.2 (0\u201324), 33.6% were male. All MM cases were metastatic and two died of disease. CMN components were negative for PRAME in all cases, whereas 3\u2009MMs and 1 PN were positive (&gt;\u200990%). MMs showed loss of H3K27me3 expression, whereas expression was retained in all PNs. Two cases showed\n                      <jats:italic>NRAS<\/jats:italic>\n                      mutations in both MM and nevus components.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Combined use of PRAME and H3K27me3 can be utilized to discriminate MM from PN arising in LCMN\/GCMN if validated on larger cohorts.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cup.70119","type":"journal-article","created":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T11:49:47Z","timestamp":1776858587000},"page":"749-755","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Diagnostic Utility of\n                    <scp>PRAME<\/scp>\n                    and\n                    <scp>H3K27me3<\/scp>\n                    in Distinguishing Malignant Melanoma From Proliferative Nodules on Congenital Melanocytic Nevi"],"prefix":"10.1111","volume":"53","author":[{"given":"Ozge","family":"Hurdogan","sequence":"first","affiliation":[{"name":"Department of Pathology, Istanbul Faculty of Medicine Istanbul University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Can","family":"Baykal","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Istanbul Faculty of Medicine Istanbul University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0130smail","family":"Yilmaz","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Health Sciences, Sultan 2. Abdul Hamid Khan Educational and Research Hospital  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Begum Yeni","family":"Erdem","sequence":"additional","affiliation":[{"name":"Department of Pathology, Istanbul Faculty of Medicine Istanbul University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nesimi","family":"Buyukbabani","sequence":"additional","affiliation":[{"name":"Department of Pathology, Istanbul Faculty of Medicine Istanbul University  Istanbul Turkey"},{"name":"Department of Pathology Koc University Hospital  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0439-5954","authenticated-orcid":false,"given":"Sule Ozturk","family":"Sari","sequence":"additional","affiliation":[{"name":"Department of Pathology, Istanbul Faculty of Medicine Istanbul University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2012.05.043"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1981.tb00954.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/pas.0000000000000351"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2013.70"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0000000000000558"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700490"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2013.429"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2008.203"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.2350\/14-10-1566-OA.1"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/dju246"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1230062"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1229259"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2014.425"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000769"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001134"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001393"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/cup.13818"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/cup.14000"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001026"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0000000000002357"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2006.06.2984"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800516"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/cup.14091"}],"container-title":["Journal of Cutaneous Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cup.70119","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cup.70119","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cup.70119","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T01:00:46Z","timestamp":1783472446000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cup.70119"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,22]]},"references-count":23,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1111\/cup.70119"],"URL":"https:\/\/doi.org\/10.1111\/cup.70119","archive":["Portico"],"relation":{},"ISSN":["0303-6987","1600-0560"],"issn-type":[{"value":"0303-6987","type":"print"},{"value":"1600-0560","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,22]]},"assertion":[{"value":"2025-09-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}