{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T02:04:34Z","timestamp":1790820274658,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cutan Pathol"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Sclerosing nevi with pseudomelanomatous features (SNPF) are rare melanocytic lesions characterized by a distinctive trizonal architecture, created by lateral shouldering of the junctional component and nests of melanocytes within the dermis that can histologically mimic melanoma, posing a diagnostic dilemma. Although prior clinicopathologic studies suggest benign behavior, their molecular features have not been characterized.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We retrospectively identified nine SNPFs (2004\u20132024) and performed detailed histopathologic and immunohistochemical evaluation. Targeted next\u2010generation sequencing (NGS) with a 498\u2010gene hybrid capture\u2010based panel was performed on microdissected, melanocyte\u2010enriched tissue, with macrodissection used as salvage in low nucleic acid yield cases that initially failed. SNPF results were compared to a comparator group of\n                      <jats:italic>BRAF<\/jats:italic>\n                      \u2010mutated melanomas tested on the same assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Patients ranged from 25 to 65\u2009years (median: 41), with lesions most commonly on the trunk. All cases demonstrated classic trizonal morphology with epidermal flattening, superficial dermal fibroplasia, and deeper dermal nevus nests. Immunohistochemistry showed uniform PRAME negativity and retained mosaic p16 expression. Molecular testing was successful in eight of the nine cases, all of which harbored a\n                      <jats:italic>BRAF<\/jats:italic>\n                      V600E mutation (VAF: 1.3%\u201327%). No additional pathogenic mutations or copy number alterations were identified. Comparator\n                      <jats:italic>BRAF<\/jats:italic>\n                      \u2010mutated melanomas had additional, detectable co\u2010occurring pathogenic mutations and\/or copy number variations in 19 of the 20 cases.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>SNPFs consistently demonstrate a molecular profile indistinguishable from conventional benign nevi and lack genomic features of melanoma. These findings provide molecular support for the benign nature of SNPF.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cup.70226","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:25:31Z","timestamp":1790817931000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Characterization of Sclerosing Nevi With Pseudomelanomatous Features"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9624-9929","authenticated-orcid":false,"given":"Zachary","family":"Corey","sequence":"first","affiliation":[{"name":"Department of Pathology University of Colorado Anschutz School of Medicine  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4065-8084","authenticated-orcid":false,"given":"Mckinzie","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Colorado Anschutz School of Medicine  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2521-9343","authenticated-orcid":false,"given":"John","family":"Fowler","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Colorado Anschutz School of Medicine  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7424-9193","authenticated-orcid":false,"given":"Joshua","family":"Wisell","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Colorado Anschutz School of Medicine  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dara L.","family":"Aisner","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Colorado Anschutz School of Medicine  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8280-527X","authenticated-orcid":false,"given":"Amy","family":"Guimaraes\u2010Young","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Colorado Anschutz School of Medicine  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3816-1542","authenticated-orcid":false,"given":"Shyam S.","family":"Raghavan","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Colorado Anschutz School of Medicine  Aurora Colorado 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