{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T06:23:23Z","timestamp":1769063003617,"version":"3.49.0"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,1,20]],"date-time":"2013-01-20T00:00:00Z","timestamp":1358640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:p>D. Demirel, N. Aky\u00fcrek and I. Ramzy \u2028<jats:bold>Diagnostic and prognostic significance of image cytometric DNA ploidy measurement in cytological samples of cervical squamous intraepithelial lesions<\/jats:bold><\/jats:p><jats:p><jats:bold>Objective:\u2002<\/jats:bold> To study the DNA ploidy pattern of uterine cervical squamous intraepithelial lesions (SILs) and its diagnostic and prognostic significance.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> The study included 31 cases of SIL: 11 low\u2010grade (LSIL) and 20 high\u2010grade (HSIL). Feulgen\u2013pararosaniline staining was performed on previously Papanicolaou\u2010stained smears and a DNA image cytometric study was performed. An internal reference was used to calibrate the samples.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> All 31 cases of SIL, either LSIL or HSIL, were non\u2010diploid. Of the 11 cases of LSIL, four were tetraploid and seven were aneuploid, whereas, of the 20 cases of HSIL, four were tetraploid and 16 were aneuploid. Stemline aneuploidy was not a significant discriminator between LSIL and HSIL (<jats:italic>P<\/jats:italic>\u2003=\u20030.32). Based on single\u2010cell analysis, HSIL cases had significantly higher DNA content than LSIL cases (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.01). When a mean of 30% or more was used for the 6c\u2010exceeding event (6cEE) value, the sensitivity and specificity to indicate HSIL were 83% and 64%, respectively, with a positive predictive value (PPV) of 81% and negative predictive value (NPV) of 65%. All HSIL cases were cervical intraepithelial neoplasia grade 2 or worse (CIN2+) on biopsy. In addition, cases which showed recurrence had more DNA content by single\u2010cell analysis than those with an indolent clinical behaviour: <jats:italic>P<\/jats:italic>\u2003=\u20030.04 and <jats:italic>P<\/jats:italic>\u2003=\u20030.03 for LSIL and HSIL, respectively.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Image cytometric DNA analysis is a useful technique for diagnostic and prognostic purposes in uterine cervical SIL when appropriate \u2018c\u2019 values are used in single\u2010cell analysis. We propose that a &gt;6c DNA content of 30% is useful as a cut\u2010off level for predicting cases with CIN2+ in DNA image cytometry of cervical smears.<\/jats:p>","DOI":"10.1111\/cyt.12039","type":"journal-article","created":{"date-parts":[[2013,1,21]],"date-time":"2013-01-21T03:04:38Z","timestamp":1358737478000},"page":"105-112","source":"Crossref","is-referenced-by-count":10,"title":["Diagnostic and prognostic significance of image cytometric DNA ploidy measurement in cytological samples of cervical squamous intraepithelial lesions"],"prefix":"10.1111","volume":"24","author":[{"given":"D.","family":"Demirel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Aky\u00fcrek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Ramzy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,20]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.23638"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.14.6622-6631.2000"},{"key":"e_1_2_7_4_2","first-page":"19","article-title":"Suppression of tumorigenesis by transcription units expressing the antisense E6 and E7 messenger RNA (mRNA) for the transforming proteins of the human papillomavirus and the sense mRNA for the retinoblastoma gene in cervical carcinoma cells","volume":"2","author":"Hu G","year":"1995","journal-title":"Cancer Gene Ther"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.11889"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.285.11.1500"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.10953"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.11010"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.20848"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1309\/6NXC-V9XD-YM87-8FAE"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.20644"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/1746-1596-6-107"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1155\/2001\/657642"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0344-0338(00)80057-6"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgi218"},{"key":"e_1_2_7_16_2","first-page":"289","article-title":"Interphase cytogenetic evidence for distinct genetic pathways in the development of squamous neoplasia of the uterine cervix","volume":"78","author":"Graham DA","year":"1998","journal-title":"Lab Invest"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(84)90043-8"},{"key":"e_1_2_7_18_2","first-page":"505","article-title":"Diagnosis of prospective malignancy in koilocytic dysplasias of the cervix with DNA cytometry","volume":"34","author":"Chatelain R","year":"1989","journal-title":"J Reprod Med"},{"key":"e_1_2_7_19_2","doi-asserted-by":"crossref","unstructured":"HanselaarAG B\u00f6ckingA GundlachHet al.International concensus conference on the fight against cervical cancer IAC Task Force 8 Summary Chicago Illinois USA. 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