{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T13:19:30Z","timestamp":1762521570150},"reference-count":14,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,2,5]],"date-time":"2013-02-05T00:00:00Z","timestamp":1360022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Cytology laboratories routinely treat cervical liquid\u2010based cytology (<jats:styled-content style=\"fixed-case\">LBC<\/jats:styled-content>) specimens that are heavily contaminated with blood with glacial acetic acid (<jats:styled-content style=\"fixed-case\">GAA<\/jats:styled-content>) in order to lyse red blood cells and facilitate assessment. However, the impact on downstream human papillomavirus (<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>) detection is not well understood. This study examines the effect of <jats:styled-content style=\"fixed-case\">GAA<\/jats:styled-content> pre\u2010treatment of ThinPrep<jats:sup>\u00ae<\/jats:sup> Preservcyt<jats:sup>\u00ae<\/jats:sup> specimens on the molecular detection of <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A panel of 150 routinely collected cervical <jats:styled-content style=\"fixed-case\">LBC<\/jats:styled-content> specimens was tested with two commercial <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> tests, the Abbott RealTime High Risk <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> test (<jats:italic>rt<\/jats:italic><jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>) and the Qiagen Hybrid Capture 2 High Risk <jats:styled-content style=\"fixed-case\">HPV DNA<\/jats:styled-content> test (<jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>2), as aliquots before and after <jats:styled-content style=\"fixed-case\">GAA<\/jats:styled-content> treatment. Statistical analysis was performed using McNemars test and Bland and Altman plots.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Agreement between the results of the <jats:italic>rt<\/jats:italic><jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> test on <jats:styled-content style=\"fixed-case\">GAA<\/jats:styled-content>\u2010untreated and <jats:styled-content style=\"fixed-case\">GAA<\/jats:styled-content>\u2010treated specimens was 95.7%, with no evidence of a significant difference in the distribution of the discrepant results (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.414). <jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>2 test agreement on <jats:styled-content style=\"fixed-case\">GAA<\/jats:styled-content>\u2010untreated and <jats:styled-content style=\"fixed-case\">GAA<\/jats:styled-content>\u2010treated specimens was 91% at a cut\u2010off of 1 relative light unit index (<jats:styled-content style=\"fixed-case\">RLUI<\/jats:styled-content>) and 92% at a cut\u2010off of 2 <jats:styled-content style=\"fixed-case\">RLUI<\/jats:styled-content>. There was no evidence of a difference in the distribution of discordant results at a cut\u2010off of 1 (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.405) and 2 <jats:styled-content style=\"fixed-case\">RLUI<\/jats:styled-content> (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.564).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">GAA<\/jats:styled-content> pre\u2010treatment of cervical ThinPrep Preservcyt <jats:styled-content style=\"fixed-case\">LBC<\/jats:styled-content> specimens had little effect on the two commercial <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> tests used in this study. The impact of <jats:styled-content style=\"fixed-case\">GAA<\/jats:styled-content> treatment on <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> testing should, however, be validated for all <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> tests and all <jats:styled-content style=\"fixed-case\">LBC<\/jats:styled-content> collection media used in each particular diagnostic setting.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12046","type":"journal-article","created":{"date-parts":[[2013,2,5]],"date-time":"2013-02-05T07:51:20Z","timestamp":1360050680000},"page":"314-320","source":"Crossref","is-referenced-by-count":9,"title":["Effect of glacial acetic acid pre\u2010treatment of cervical liquid\u2010based cytology specimens on the molecular detection of human papillomavirus"],"prefix":"10.1111","volume":"24","author":[{"given":"C.","family":"Moore","sequence":"first","affiliation":[{"name":"Scottish HPV Reference Laboratory Royal Infirmary of Edinburgh Edinburgh UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Cuschieri","sequence":"additional","affiliation":[{"name":"Scottish HPV Reference Laboratory Royal Infirmary of Edinburgh Edinburgh UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"McQueen","sequence":"additional","affiliation":[{"name":"Pathology Department Royal Infirmary of Edinburgh Edinburgh UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Duvall","sequence":"additional","affiliation":[{"name":"Pathology Department Royal Infirmary of Edinburgh Edinburgh UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Graham","sequence":"additional","affiliation":[{"name":"Epidemiology and Statistics Core Wellcome Trust Clinical Research Facility Edinburgh UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. A.","family":"Cubie","sequence":"additional","affiliation":[{"name":"Scottish HPV Reference Laboratory Royal Infirmary of Edinburgh Edinburgh UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,2,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1309\/GE86-156K-EGM2-DGXB"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21349"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06719-11"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(09)70007-1"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20152"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20437"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21425"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20890"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP8N9ZJQDHFYTC"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-010-1556-4"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.21271"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.d2757"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00896-09"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.d2941"}],"container-title":["Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcyt.12046","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cyt.12046","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T21:21:21Z","timestamp":1696281681000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cyt.12046"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,2,5]]},"references-count":14,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2013,10]]}},"alternative-id":["10.1111\/cyt.12046"],"URL":"https:\/\/doi.org\/10.1111\/cyt.12046","archive":["Portico"],"relation":{},"ISSN":["0956-5507","1365-2303"],"issn-type":[{"type":"print","value":"0956-5507"},{"type":"electronic","value":"1365-2303"}],"subject":[],"published":{"date-parts":[[2013,2,5]]}}}