{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,11]],"date-time":"2026-01-11T16:23:00Z","timestamp":1768148580907,"version":"3.49.0"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,11,21]],"date-time":"2013-11-21T00:00:00Z","timestamp":1384992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the association of human papillomavirus (<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>) 16 and non\u201016 genotype, p16\/Ki\u201067 dual staining and koilocytosis and their role in the prediction of the clinical outcome of low\u2010grade squamous intraepithelial lesion (<jats:styled-content style=\"fixed-case\">LSIL<\/jats:styled-content>) cytology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>One hundred and fifty\u2010five patients with <jats:styled-content style=\"fixed-case\">LSIL<\/jats:styled-content> were followed up and recorded as progression, persistence or regression. <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> genotyping was performed for high\u2010risk <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> (hr<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>) <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010positive cases. Koilocytosis was reviewed and p16\/Ki\u201067 dual staining was performed on reprocessed conventional cytology slides.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>16 was the most frequent genotype found in 16.3% of cases. p16\/Ki\u201067 dual staining was positive in 36.1% of all cases. Progression, including concurrent cervical intraepithelial lesion grade 2 or above (<jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>2+), was recorded in 13.8% of cases. A statistically significant difference between progressive and non\u2010progressive cases was shown by the following: hr<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010positive versus hr<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010negative (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.022), <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>16\u2010positive versus non\u201016 <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010positive (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001) and p16\/Ki\u201067\u2010positive versus p16\/Ki\u201067\u2010negative (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001) cases. Cases with combined <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>16 and p16\/Ki\u201067 positivity showed the highest progression rate (58.3%). Non\u2010koilocytic <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>16\u2010positive cases showed a 50% progression rate compared with 10.1% for koilocytic non\u201016 <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010positive cases (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.010). The sensitivity of p16\/Ki\u201067 dual staining for the detection of <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>2+ lesions was 80%, comparable with hr<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> (85%). The specificity of p16\/Ki\u201067 dual staining was 71% and of hr<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> 42%. The highest specificity was found for <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>16 genotype presence (91%), but with low sensitivity (50%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> genotyping, p16\/Ki\u201067 dual staining and koilocytic morphology can be useful in the prediction of clinical outcome in women initially diagnosed with <jats:styled-content style=\"fixed-case\">LSIL<\/jats:styled-content> cytology.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12121","type":"journal-article","created":{"date-parts":[[2013,11,22]],"date-time":"2013-11-22T02:10:35Z","timestamp":1385086235000},"page":"10-18","source":"Crossref","is-referenced-by-count":19,"title":["<scp>HPV<\/scp>16 genotype, p16\/Ki\u201067 dual staining and koilocytic morphology as potential predictors of the clinical outcome for cervical low\u2010grade squamous intraepithelial lesions"],"prefix":"10.1111","volume":"26","author":[{"given":"D.","family":"Vrdoljak\u2010Mozeti\u010d","sequence":"first","affiliation":[{"name":"Department of Clinical Cytology University of Rijeka Rijeka Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Kra\u0161evi\u0107","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Rijeka  Rijeka Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Ver\u0161a Ostoji\u0107","sequence":"additional","affiliation":[{"name":"Department of Clinical Cytology University of Rijeka Rijeka Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"\u0160temberger\u2010Papi\u0107","sequence":"additional","affiliation":[{"name":"Department of Clinical Cytology University of Rijeka Rijeka Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Rube\u0161a\u2010Mihaljevi\u0107","sequence":"additional","affiliation":[{"name":"Department of Clinical Cytology University of Rijeka Rijeka Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Bubonja\u2010\u0160onje","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Clinical Hospital Centre Rijeka Faculty of Medicine University of Rijeka Rijeka Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,11,21]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.5858\/2004-128-1224-BIARRP","article-title":"Bethesda 2001 implementation and reporting rates","volume":"128","author":"Davey DD","year":"2004","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6602069"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0029-7844(98)00245-2"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djg037"},{"key":"e_1_2_5_6_1","first-page":"1763","article-title":"Natural history of established low grade cervical intraepithelial (CIN1) lesions","volume":"28","author":"Bansal N","year":"2008","journal-title":"Anticancer Res"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2008.00623.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/LGT.0b013e318287d329"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21664"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.b2546"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20172"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1258\/ijsa.2011.011336"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4612-2042-8"},{"key":"e_1_2_5_14_1","first-page":"505","article-title":"Condylomatous lesions of the cervix and vagina. 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