{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T04:49:34Z","timestamp":1778820574919,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,4,28]],"date-time":"2014-04-28T00:00:00Z","timestamp":1398643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Konkuk University Medical Center","award":["Research Grant 2012"],"award-info":[{"award-number":["Research Grant 2012"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study was performed to evaluate the prognostic significance of human papillomavirus (<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>) viral load, expressed in relative light units (<jats:styled-content style=\"fixed-case\">RLU<\/jats:styled-content>s), in patients with atypical squamous cells of undetermined significance (<jats:styled-content style=\"fixed-case\">ASC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">US<\/jats:styled-content>) cytology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 349 <jats:styled-content style=\"fixed-case\">ASC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">US<\/jats:styled-content> cases with <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> infection, detected using Hybrid Capture 2, were diagnosed histologically. A colposcopically directed punch biopsy was performed on acetowhite areas. Endocervical curettage biopsy and random cervical punch biopsy in four quadrants were performed in unsatisfactory colposcopy cases. In negative colposcopy cases, random cervical punch biopsy in four quadrants was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Case with no cervical intraepithelial neoplasia (<jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>), <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>2+ (<jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>2\/<jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>3) accounted for 162, 135 and 52 cases, respectively. The mean age showed no difference among the three groups (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.510). There was a significant correlation between <jats:styled-content style=\"fixed-case\">RLU<\/jats:styled-content> values and the presence of <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content> (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.001), but less so with its severity: the median <jats:styled-content style=\"fixed-case\">RLU<\/jats:styled-content> values for negative, <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>2+ cases were 42.68, 146.45 and 156.43, respectively, with widely overlapping confidence intervals. The cut\u2010off values of <jats:styled-content style=\"fixed-case\">RLU<\/jats:styled-content> to detect <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>1+ and <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>2+ were 6.73 and 45.64, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> viral load in <jats:styled-content style=\"fixed-case\">ASC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">US<\/jats:styled-content> cases showed a significant correlation with the presence of <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content> and less so with its severity, and showed large overlap of viral loads between grades of <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>. In <jats:styled-content style=\"fixed-case\">ASC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">US<\/jats:styled-content> cases, <jats:styled-content style=\"fixed-case\">RLU<\/jats:styled-content> was not an accurate predictor of immediate high\u2010grade <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12146","type":"journal-article","created":{"date-parts":[[2014,4,28]],"date-time":"2014-04-28T06:48:47Z","timestamp":1398667727000},"page":"19-25","source":"Crossref","is-referenced-by-count":8,"title":["Semi\u2010quantitative HPV viral load in patients with <scp>ASC<\/scp>\u2010<scp>US<\/scp> cytology: viral load correlates strongly with the presence of <scp>CIN<\/scp> but only weakly with its severity"],"prefix":"10.1111","volume":"26","author":[{"given":"S. J.","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Konkuk University Hospital Konkuk University School of Medicine Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. Y.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pathology Konkuk University Hospital Konkuk University School of Medicine Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.\u2010H.","family":"Shim","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Konkuk University Hospital Konkuk University School of Medicine Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.\u2010H.","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Konkuk University Hospital Konkuk University School of Medicine Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. K.","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Konkuk University Hospital Konkuk University School of Medicine Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. S.","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Pathology Konkuk University Hospital Konkuk University School of Medicine Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.\u2010N.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Konkuk University Hospital Konkuk University School of Medicine Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.\u2010B.","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Konkuk University Hospital Konkuk University School of Medicine Seoul South 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